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Plasmid#117362PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR SEMA5B-202
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-SEMA5B-211
Plasmid#117363PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR SEMA5B-211
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-RPLP1-201
Plasmid#117361PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR RPLP1-201
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-ASIC1
Plasmid#117339PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR ASIC1
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-ARF1-214
Plasmid#117337PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR ARF1-214
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-LEMD2-201
Plasmid#117349PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR LEMD2-201
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-PPM1F-201
Plasmid#117334PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR PPM1F-201
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-GLIS2-201
Plasmid#117331PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR GLIS2-201
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-CERS2-201
Plasmid#117330PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR CERS2-201
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-M4
Plasmid#114036PurposeExpression of HEC1 M4 mutant tagged with GFPDepositorInsertGFP tagged - HEC1 -M4 (NDC80 Human)
UseTagsExpressionMammalianMutationM4 (K146E, R153E, K156E)PromoterCMV TetOAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-M2
Plasmid#114035PurposeExpression of HEC1 M2 mutant tagged with GFPDepositorInsertGFP tagged - HEC1 -M2 (NDC80 Human)
UseTagsExpressionMammalianMutationM2 (K81E, N87E, K89E)PromoterCMV TetOAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1 PUM FL
Plasmid#112997PurposeFor protein expression and purification of Drosophila pumilio (amino acids 566-1090)DepositorInsertpumilio (pum Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAT_g1 gRNA
Plasmid#86005Purposeplasmid vector encoding for U6-driven AAT_g1 gRNADepositorInsertpU6-AAT_g1 sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterpU6Available sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAT_g2 gRNA
Plasmid#86006Purposeplasmid vector encoding for U6-driven AAT_g2 gRNA (Z-allele specific)DepositorInsertpU6-AAT_g2 sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hmga2 shMut wt
Plasmid#52036Purposeencodes shMut HMGA2DepositorInsertHmga2 (Hmga2 Mouse)
UseTagsExpressionMammalianMutationshRNA binding site mutatedPromoterCMVAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER dCas9-TET1CDMut
Plasmid#101920PurposeThe dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.DepositorInsertdCas9-TET1CD Mut
UseLentiviralTagsExpressionMammalianMutationMutated TET1 catalytic domainPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGD-p19
Plasmid#196326Purpose35S promoter-driven expression of the tomato bushy stunt virus p19 RNA silencing suppressorDepositorInserttomato bushy stunt virus p19
UseTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Artichoke
Plasmid#73320PurposeArtichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)DepositorTypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianMutationPromoterSFFV and EF1aAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDNR-SpCas9-Gem
Plasmid#80424PurposeUsed for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joiningDepositorInsertSpCas9-Gem
UseCRISPRTagsGemininExpressionMutationcodon optimized for humanPromoterT7Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1
Plasmid#193942Purposea fully murine CAR targeting CD105 (endoglin), a component of the TGFβ receptor expressed on the surface of certain solid tumors and acute leukemias.DepositorInsertheavy- and light-chain regions of the MJ7/18 hybridoma and constructed a single chain variable fragment (scFv)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only