Addgene

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pAAV-EF1a-vCre

Plasmid

#55638

Purpose

Expresses vCre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde and AAV8

Insert

vCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO-RB1-shRNA19

Plasmid

#25640

Purpose

3rd generation lentiviral vector

Depositor

Todd Waldman

Article

Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

Insert

RB1-shRNA 19 (RB1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mG2a_M18

Plasmid

#105930

Purpose

Expression plasmid coding for heavy chain of mouse IgG2a antibody specific to antigen B of the ABO blood group system, clone M18.

Depositor

Joanna Bereta

Article

Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

Insert

IgG2b heavy chain (Ighg2b Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

hEF1-HTLV prom

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-OC43-Hemagglutinin-esterase

Plasmid

#168943

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-Hemagglutinin-esterase (HE )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO-RB1-shRNA63

Plasmid

#25641

Purpose

3rd generation lentiviral vector

Depositor

Todd Waldman

Article

Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

Insert

RB1-shRNA 63 (RB1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Akt-FoxO3a-KTR-EGFP

Plasmid

#125131

Purpose

Fluorescent reporter for Akt activity

Depositor

Kazuhiro Aoki

Article

Maryu et al Cell Struct Funct. 2016 Jul 22;41(2):81-

Insert

FOXO2 (FOXO3 Human)

Use

Tags

Enhanced green fluorescent protein (EGFP)

Expression

Mammalian

Mutation

FoxO3A 1-402 amino acid

Promoter

CAG promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ131-STU-Lb

Plasmid

#138096

Purpose

Golden Gate entry vector for 1st crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ134-STU-Lb

Plasmid

#138105

Purpose

Golden Gate entry vector for 4th crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ133-STU-Lb

Plasmid

#138102

Purpose

Golden Gate entry vector for 3rd crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ132-STU-Lb

Plasmid

#138099

Purpose

Golden Gate entry vector for 2nd crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pMJ179

Plasmid

#85996

Purpose

One-guide Perturb-seq vector backbone; modified mouse U6 promoter; constant region 2

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

EGFP-NT2_cr2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

modified mouse U6

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shRNA beta-catenin

Plasmid

#18803

Purpose

3rd gen lentiviral vector for knocking down beta-catenin gene expression

Depositor

Bob Weinberg

Article

Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

Insert

b-catenin shRNA (CTNNB1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 22, 2008

Availability

Academic Institutions and Nonprofits only

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pMJ117

Plasmid

#85997

Purpose

One-guide Perturb-seq vector backbone; modified human U6 promoter; constant region 3

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

EGFP-NT2_cr3
puro-T2A-BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

modified human U6

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1Xpress-BcrGAP

Plasmid

#38175

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR (BCR Human)

Use

Tags

Xpress

Expression

Mammalian

Mutation

contains GAP domain amino acids 1004-1271

Promoter

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3.1Xpress-AbrGAP

Plasmid

#38177

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

Abr GAP domain (ABR Human)

Use

Tags

Xpress

Expression

Mammalian

Mutation

ABR GAP domain including amino acids 597-859

Promoter

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
pCSII-EF-miRFP670v1-hGem(1/110)

Plasmid

#80006

Purpose

hGem(1/110) fused with near-infrared fluorescent protein miRFP670v1 (S/G2/M phase cell cycle reporter)

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

hGeminin (1/110) (GMNN Human)

Use

Lentiviral

Tags

miRFP670v1

Expression

Mutation

Promoter

EF-1 alpha

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ144-ZmUbi-pT

Plasmid

#138108

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs driven by Zea mays Ubi promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pMito-miRFP703

Plasmid

#80000

Purpose

Mitochondria labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

cytochrome C oxidase subunit VIII (COX8A Human)

Use

Tags

miRFP703

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 HA-hTSPAN12

Plasmid

#115784

Purpose

Expresses human TSPAN12 with N-terminal HA-tag in mammalian cells. Contains multiple silent mutations that introduce restriction sites.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

TSPAN12 (TSPAN12 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

multiple silent mutations introduce restriction s…

Promoter

CMV

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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6His-MBP-TEV-huLbCpf1

Plasmid

#90096

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huLbCpf1

Use

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Mutation

Promoter

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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