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Showing: 8381 - 8400 of 48639 results

  1. gCBEv2

    Plasmid
    #220617
    Purpose
    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv2) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    et al Nat Commun 15
    Insert
    hUNG2-nSpCas9
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220617-plasmid-map-sequence-id-436955

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  7. pDonor_RB-TnV2

    Plasmid
    #213907
    Purpose
    Barcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sites
    Depositor
    James Collins
    Article
    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.
    Insert
    mariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    213907-plasmid-map-sequence-id-422622

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  8. pDonor_RB-TnV2_pJEx

    Plasmid
    #213908
    Purpose
    Barcoded mariner transposon with crystal violet-inducible outward promoter - barcodes can be added via FseI-SbfI restriction sites
    Depositor
    James Collins
    Article
    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.
    Insert
    mariner transposon with kan resistance and EilR/pJEx and FseI-SbfI sites for barcode insertion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    213908-plasmid-map-sequence-id-422595

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  10. pSLS.544

    Plasmid
    #184961
    Purpose
    Test effect of a1/a2 length on editing murF using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 22
    Use
    Tags
    Expression
    Bacterial
    Mutation
    murF donor G1359A, a1/a2 length extended to 22 bp
    Promoter
    T7/lac
    Available since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184961-plasmid-map-sequence-id-376648

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  17. Pb mD3S4-dN

    Plasmid
    #180252
    Purpose
    P. berghei D3 (502-617) construct with N516T, S533N and N539Q mutations to abolish N-glycosylation was used for crystallization.
    Depositor
    Timothy Springer
    Article
    Feng et al Elife. 2021 Dec 23;10. pii: 74707. doi:
    Insert
    D3 of P. berghei HAP2 with N-glycosylation sites mutated
    Use
    Tags
    6His
    Expression
    Mammalian
    Mutation
    N516T, S533N and N539Q
    Promoter
    Available since
    March 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    180252-plasmid-map-sequence-id-357204

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Showing: 8381 - 8400 of 48639 results