-
Plasmid#72924PurposeGateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human S37A-GATA6DepositorInsertGATA Binding Protein 6 (GATA6 Human)
UseEntry vector for gateway cloningTags3XAU1 and 3XFLAGExpressionBacterialMutationSerine#37 changed to Alanine;Silent mutations mad…PromoterTRE tightAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLIKneo 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant
Plasmid#72617PurposeExpresses an inducible and RNAi resistant, N-terminally 3X-FLAG tagged, C-terminally 3X-AU1 tagged S37AGATA6DepositorInsertGATA Binding Protein 6 (GATA6 Human)
UseLentiviralTags3XAU1 and 3XFLAGExpressionMammalianMutationSerine#37 changed to Alanine;Silent mutations mad…PromoterTRE tightAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-mOC-STAMP (N162D)-GFP
Plasmid#73580PurposeLentivirus expression of mOC-STAMP with N162D mutationDepositorInsertOC-STAMP (Ocstamp Mouse)
UseLentiviralTagsGFPExpressionMutationchanged asparagin (N) 162 to Aspartic Acid (D)PromoterAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
mOC-STAMP (N162D)_3xFlag-Myc-CMV26
Plasmid#73578PurposeExpress mouse OC-STAMP ((N162D) in mammalian cellsDepositorInsertOC-STAMP (Ocstamp Mouse)
UseTags3x Flag and c-Myc tagExpressionMammalianMutationchanged asparagin (N) 162 to Aspartic Acid (D)PromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRFP shRNA-resistant TrkB F616A -S478A
Plasmid#72718PurposeMammalian expression of TrkB fused to RFP in C-terminus, shRNA resistant and lacking TIAM1-binding siteDepositorInsertTrkB
UseTagsRFPExpressionMammalianMutationshRNA-resistant TrkB (T300C, T301A, C302G, C306A,…PromoterCMVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Anti-Ankyrin-G (staining) [N106/36R-rat IgG2a]
Plasmid#225416PurposeMammalian Expression Plasmid of anti-Ankyrin-G (staining) (Human) rat IgG2a R-mAb. Derived from hybridoma N106/36.DepositorInsertAnti-Ankyrin-G (staining) (Homo sapiens) recombinant (Mouse) monoclonal antibody. (ANK3 Chimera: M. musculus (mouse)/R. norvegicus (rat))
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Hygro-amp-sgRNA-resistant PBRM1
Plasmid#107407PurposeLentiviral expression of gRNA resistant PBRM1DepositorInsertsg resistant PBRM1 (PBRM1 Human)
UseLentiviralTagsExpressionMutationsgRNA resistantPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Mammalian expression: Z-lock cofilin dark state mutant
Plasmid#141136Purposea control for optogenetic cofilin analogDepositorInsertZdk2, Cofilin, LOV2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4 h-Stx3 resistant to shRNA #304-2xMyc/His
Plasmid#99746PurposeExpresses human Stx3 with a C-term myc-myc-his that is resistant to shRNA #304 from Sigma Aldrich.DepositorInsertStx3 (STX3 Human)
UseTagsmyc-myc-hisExpressionMammalianMutationMade six (6) silent mutations to confer resistanc…PromoterCMV/TOAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTS97 pHAGE2 CMVtetO2 GFP-22xGS-SUMOstar-MCS
Plasmid#199350PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOstar-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFP-SUMOstarExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS108 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-GFP
Plasmid#199361PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTags3C-SUMOstar-EGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Chx10 (2.5kb distal 5' reg)+SV40p-tdTomato-IRES-AP
Plasmid#18814DepositorInsertChx10 (2.5kb distal 5' reg)+SV40p-tdTomato-IRES-AP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
JI401: Ad5 E1A/Hexon qRT-PCR standard
Plasmid#131753PurposePlasmid standard containing regions of the Ad5 E1A and Ad5 Hexon genes to create a qRT-PCR standard curve for detection of replication competent adenovirus (RCA).DepositorInsertsAd5 E1A gene fragment
Ad5 Hexon gene fragment
UseStandard for qrt-pcrTagsExpressionMutationPromoterN/AAvailable sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pALPS_3'LTR GFP@gag start SFFV rev
Plasmid#101339PurposeLTR drives GFP and internal SFFV promoter drives codon optimized revDepositorInsertGFP, rev
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
E. coli expression: Z-lock cofilin dark state mutant
Plasmid#141135Purposea control for optogenetic cofilin analogDepositorInsertZdk2, Cofilin, LOV2
UseTagsExpressionBacterialMutationD3 deletion, I32F in Zdk2PromoterAvailable sinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pALPS_3'LTR GFP@gag start SFFV tat
Plasmid#101338PurposeLTR drives GFP and internal SFFV promoter drives codon optimized tatDepositorInsertGFP, tat
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG
Plasmid#200792PurposeMammalian expression of CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAGDepositorInsertKIF5A (Kif5a Mouse)
UseTagsCLIPf and SNAPf, HisTag, FLAGExpressionMammalianMutationΔ954-1027PromoterCMV & T7Available sinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG
Plasmid#200798PurposeMammalian expression of CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAGDepositorInsertKIF5A (Kif5a Mouse)
UseTagsCLIPf and SNAPf, HisTag, FLAGExpressionMammalianMutation411-680PromoterCMV & T7Available sinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-TRIP8b (constant) ion channel [N212/27R]
Plasmid#220409PurposeMammalian Expression Plasmid of anti-TRIP8b (constant) ion channel (Rat) IgG2a R-mAb. Derived from hybridoma N212/27.DepositorInsertAnti-TRIP8b (constant) ion channel (Rattus novegicus) recombinant mouse monoclonal antibody. (Pex5l Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-609 C-Tag Venus210[2] L20 Stat3 mCherry
Plasmid#130084PurposePPI (BiFC/TriFC) assay vectorDepositorInsertStat3 (Stat3 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only