Skip to main content
Addgene
Showing: 8361 - 8380 of 53137 results

  3. R620-M18-303: CMV51p> SARS-CoV-2 S-RBD(319-541)-His6 K417T/E484K/N501Y

    Plasmid
    #170202
    Purpose
    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.28 variant
    Depositor
    Dominic Esposito
    Article
    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.
    Insert
    SARS-CoV-2 RBD (S SARS-CoV-2)
    Use
    Tags
    His6
    Expression
    Mammalian
    Mutation
    K417T, E484K, N501Y
    Promoter
    CMV51
    Available since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170202-plasmid-map-sequence-id-334271

    Enlarge

  7. R620-M06-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP K417N/E484K/N501Y

    Plasmid
    #170192
    Purpose
    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), triple mutant from B.1.351 variant
    Depositor
    Dominic Esposito
    Article
    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.
    Insert
    SARS-CoV-2 RBD (S SARS-CoV-2)
    Use
    Tags
    3C-His8-SBP
    Expression
    Mammalian
    Mutation
    K417N, E484K, N501Y
    Promoter
    CMV51
    Available since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170192-plasmid-map-sequence-id-334252

    Enlarge

  9. pTM-RBDv2

    Plasmid
    #162785
    Purpose
    SARS-CoV-2 S protein receptor binding domain (RBD) expression in mammalian cells
    Depositor
    Ivan Vorobiev
    Article
    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi
    Insert
    SARS-CoV-2 S protein receptor binding domain (RBD) (S Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
    Use
    Tags
    10xHis, c-myc, and hTPA leader
    Expression
    Mammalian
    Mutation
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162785-plasmid-map-sequence-id-333799

    Enlarge

  13. R620-M19-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP K417T/E484K/N501Y

    Plasmid
    #170203
    Purpose
    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.28 variant
    Depositor
    Dominic Esposito
    Article
    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.
    Insert
    SARS-CoV-2 RBD (S SARS-CoV-2)
    Use
    Tags
    3C-His8-SBP
    Expression
    Mammalian
    Mutation
    K417T, E484K, N501Y
    Promoter
    CMV51
    Available since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170203-plasmid-map-sequence-id-334219

    Enlarge

  15. R620-M05-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 K417N/E484K/N501Y

    Plasmid
    #170191
    Purpose
    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), triple mutant from B.1.351 variant
    Depositor
    Dominic Esposito
    Article
    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.
    Insert
    SARS-CoV-2 RBD (S SARS-CoV-2)
    Use
    Tags
    His6
    Expression
    Mammalian
    Mutation
    K417N, E484K, N501Y
    Promoter
    CMV51
    Available since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170191-plasmid-map-sequence-id-334254

    Enlarge

  18. pQ-Elo-HB-C-A(1-630)

    Plasmid
    #171086
    Purpose
    Co-expresses of ΔC-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Deleted N-term 26aa and C-term 142aa for mutant e…
    Promoter
    T5 promoter
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171086-plasmid-map-sequence-id-335555

    Enlarge

  19. pQ-Elo-HB-C-A(400-772)

    Plasmid
    #171087
    Purpose
    Co-expresses of ΔN-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Deleted N-term 26+399 amino acids for mutant expr…
    Promoter
    T5 promoter and T7 promoter
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171087-plasmid-map-sequence-id-335508

    Enlarge

  20. pQ-Elo-HB-C-A(1-772)

    Plasmid
    #171085
    Purpose
    Co-expresses of WT-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Deleted N-term 26 amino acids for expression purp…
    Promoter
    T5 promoter
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171085-plasmid-map-sequence-id-335501

    Enlarge

Showing: 8361 - 8380 of 53137 results