Addgene

We narrowed to 13,599 results for: pET

Showing: 8321 - 8340 of 13599 results

Results per page:

Sort results by:

pAc5.1B-EGFP-DmPAN2-del246-249-dsRNAres_Y

Plasmid

#148034

Purpose

Insect Expression of DmPAN2-del246-249-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-del246-249-dsRNAres (PAN2 Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmPAN2-del358-467-T97RH102G-dsRNAres_Y

Plasmid

#148036

Purpose

Insect Expression of DmPAN2-del358-467-T97RH102G-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-del358-467-T97RH102G-dsRNAres (PAN2 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmCYFIP1_T

Plasmid

#147578

Purpose

Insect Expression of DmCYFIP1

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmCYFIP1 (Sra-1 Fly)

Use

Tags

Expression

Insect

Mutation

5 silent and one non silent A146V mutations compa…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmCYFIP1_T

Plasmid

#147585

Purpose

Insect Expression of DmCYFIP1

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmCYFIP1 (Sra-1 Fly)

Use

Tags

Expression

Insect

Mutation

5 silent and one non silent A146V mutations compa…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmCYFIP1-4xmut_T

Plasmid

#147586

Purpose

Insect Expression of DmCYFIP1-4xmut

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmCYFIP1-4xmut (Sra-1 Fly)

Use

Tags

Expression

Insect

Mutation

5 silent and one non silent A146V mutations compa…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmCYFIP1-K743E_T

Plasmid

#147587

Purpose

Insect Expression of DmCYFIP1-K743E

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmCYFIP1-K743E (Sra-1 Fly)

Use

Tags

Expression

Insect

Mutation

5 silent and one non silent A146V mutations compa…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmeIF4Ga_578-650-CG32016-E_T

Plasmid

#147588

Purpose

Insect Expression of DmeIF4Ga_578-650-CG32016-E

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmeIF4Ga_578-650-CG32016-E (eIF4G1 Fly)

Use

Tags

Expression

Insect

Mutation

CG32016-E 5 silent and three no silent mutations …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmThor-V5His6_G

Plasmid

#146398

Purpose

Insect Expression of DmThor

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor (Thor Fly)

Use

Tags

Expression

Insect

Mutation

two silent mutations compared to the sequence giv…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-CvH-HseIF4E_24-217_AF

Plasmid

#148668

Purpose

Bacterial Expression of HseIF4E_24-217

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4E_24-217 (EIF4E Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
2xNLS-NLP-cMyc AspCas12a

Plasmid

#182120

Purpose

pET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

2xNLS-NLP-cMyc AspCas12a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
H2B-mNeonGreen_FRB(DmrC)_IRESpuro2

Plasmid

#182918

Purpose

Expesses H2B-mNeonGreen_FRB(DmrC). Can be used to target FKBP(DmrA) tagged proteins to H2B

Depositor

Daniel Gerlich

Article

Cuylen-Haering et al Nature. 2020 Nov;587(7833):285-290. doi:

Insert

pH2B (H2BC16P Human)

Use

Tags

mNeonGreen-FRB(DmrC)

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgNT.3#3/Cre

Plasmid

#173662

Purpose

Expresses a NT.3-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

Non-targeting gRNA

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.U6gT28.4.hSyn.flex.H2B.RFP

Plasmid

#170386

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Cre-inducible expression of nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

Cre inducible H2B RFP

Use

AAV

Tags

Expression

Mutation

Promoter

human Synapsin

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.U6gT28.3.hSyn.flex.H2B.RFP

Plasmid

#170374

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Cre-inducible expression of nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

Cre inducible H2B RFP

Use

AAV

Tags

Expression

Mutation

Promoter

human Synapsin

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.U6gT28.13.hSyn.flex.H2B.RFP

Plasmid

#170376

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Cre-inducible expression of nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

Cre inducible H2B RFP

Use

AAV

Tags

Expression

Mutation

Promoter

human Synapsin

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-U6-UL29-U3-UL8

Plasmid

#166685

Purpose

To express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (non-EGFP version).

Depositor

Yujia Cai

Article

Yin et al Nat Biotechnol. 2021 Jan 11. pii: 10.103

Insert

UL8 and UL29 gRNA (UL29, UL8 HSV-1)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3HA-AMOT p130∆PPxY1(L106P)

Plasmid

#166714

Purpose

expresses AMOT(L106P) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

Angiomotin p130 L106P (AMOT Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

changed leucine 106 to proline

Promoter

CMV promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3HA-AMOT p130∆PPxY1(A112K)

Plasmid

#166715

Purpose

expresses AMOT(A112K) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

Angiomotin p130 A112K (AMOT Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

changed alanine 112 to lysine

Promoter

CMV promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEMS2155

Plasmid

#141065

Purpose

AAV plasmid with SYN1 promoter driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Galvan et al Hum Gene Ther. 2021 Apr 16. doi: 10.1089

Insert

SYN1-EmGFP-WPRE

Use

AAV

Tags

Expression

Mutation

Promoter

human Synapsin

Available since

April 28, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pRS313_ATP3G919C

Plasmid

#120255

Purpose

Expression of ATP3-7 in yeast

Depositor

Markus Ralser

Article

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

G919 changed to C; Alanine 307 changed to Proline

Promoter

ATP3

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge