Showing: 8321 - 8340 of 16096 results
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Plasmid#220463PurposeExpress S387 phosphomimetic mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cellsDepositorInsertHuman AGO2 (AGO2 Human)
UseTags3xFLAGExpressionMammalianMutationSerine 387 to aspartatePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pBAD-bHs
Plasmid#134259PurposeFor L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tagDepositorInsertHyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
UseTags6x HisExpressionBacterialMutationcodon optimized for Salmonella typhimuriumPromoterAraCAvailabilityAcademic Institutions and Nonprofits only -
pKP134 [pRS406-YBR139Wp-YBR139W(S219,D415,H474A)-GFP-ADH1t]
Plasmid#106469PurposeExpresses Atg42/Ybr139w(S219,D415,H474A) with a C-terminal GFP tag in yeast cellsDepositorInsertYBR139W(S219,D415,H474A)
UseTagsGFPExpressionBacterial and YeastMutationChanged Serine 219, Aspartate 415 and Histidine 4…PromoterYBR139WAvailabilityAcademic Institutions and Nonprofits only -
pLXSN p110 CUX1
Plasmid#90471PurposeRetroviral vector expressing human p110 CUX1 (amino acids 747-1505) with a Myc and HA tag at the N- and C-terminue, respectivelyDepositorInsertCUX1 (amino acids 747-1505) (CUX1 Human)
UseRetroviralTagsHA and MycExpressionMammalianMutationPromoterMoloney murine leukemia virus long terminal repeatAvailabilityAcademic Institutions and Nonprofits only -
Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605
Plasmid#108362PurposeExpression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegansDepositorInsertsUseTags2xFlag, MYC, T2A peptide, and TY1 linkerExpressionWormMutationPromoterA rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…AvailabilityAcademic Institutions and Nonprofits only -
pT2iApowriter2(#336)
Plasmid#184104Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenicDepositorInsertmyr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transient transgenesisTagsinternal tag: 5mycExpressionMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …PromoterZf-UbiAvailabilityAcademic Institutions and Nonprofits only -
pT22Apowriter2(#340)
Plasmid#184105Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenicDepositorInsertmyr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transgenesis (blue eyes)Tagsinternal tag: 5mycExpressionMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …PromoterZf-UbiAvailabilityAcademic Institutions and Nonprofits only -
pT2iU6Apowriter2N(#355)
Plasmid#184106Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesisDepositorInsertmyr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transient transgenesis + in vitro trans…Tagsinternal tag: 5mycExpressionMutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …PromoterZf-Ubi+SP6AvailabilityAcademic Institutions and Nonprofits only -
pMMS22L-siR3
Plasmid#221013Purposemammalian expression vector of myc-Flag tagged MMS22L siRNA resistantDepositorInsertMMS22L (MMS22L Human)
UseTagsFlag and mycExpressionMammalianMutationsilent mutations MMS22L: 5'-aaAacGtgTtgCtgTg…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pUDP082
Plasmid#103875PurposeBroad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianusDepositorInsertHH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus
UseCRISPRTagsExpressionYeastMutationPromoterScTDH3AvailabilityAcademic Institutions and Nonprofits only -
pMADM-alpha
Plasmid#36890DepositorInsertsGFP
tdTomato-3Myc
beta-globin intron
Neo
ATG (T)
GFP
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, deleted…PromoterCAG (chicken beta actin promoter and CMV enhancer…AvailabilityAcademic Institutions and Nonprofits only -
pUDE735
Plasmid#103024Purposeexpression of a Cpf1 programming crRNA targetting CAN1, HIS4, PDR12 and ADE2 (crCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S)DepositorInsertcrCAN1-4.crHIS4-4.crPDR12-3.crADE2-3.S
UseCRISPRTagsExpressionYeastMutationPromoterSNR52AvailabilityAcademic Institutions and Nonprofits only -
pET21a.Tat(48-57)-I-SceI
Plasmid#165041PurposeExpression of Tat(48-57) conjugated I-SceI for protein purification (6XHis tag)DepositorInsertI-SceI
UseTags6XHis and Tat(48-57)ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Vph1-2GFP-URA
Plasmid#207009PurposeIntegration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.DepositorInsertVPH1
UseTags2xGFPExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Ypq2-2GFP-URA
Plasmid#207010PurposeIntegration of 2xGFP tag at YPQ2 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.DepositorInsertYPQ2
UseTags2xGFPExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
EMC1-2EGFP-ter-NAT
Plasmid#184763PurposeMarker for yeast endoplasmic reticulum (ER). Integration of 2xGFP tag at EMC1 C terminus. Uses antibiotic resistance marker natMX6.DepositorInsertEMC1 (EMC1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
SEC7-2EGFP-ter-NAT
Plasmid#184764PurposeMarker for yeast late Golgi/early endosome . Integration of 2xGFP tag at SEC7 C terminus. Uses antibiotic resistance marker natMX6.DepositorInsertSEC7 (SEC7 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
IRE1-2EGFP-ter-kanMX4
Plasmid#184766PurposeIntegration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4.DepositorInsertIRE1 (IRE1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
CIT1-2EGFP-ter-URA3
Plasmid#184767PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at CIT1 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorInsertCIT1 (CIT1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
ABF2-2EGFP-ter-URA3
Plasmid#184768PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at ABF2 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorInsertABF2 (ABF2 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 8321 - 8340 of 16096 results