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Plasmid#214606PurposeExpresses reversibly switchable fluorescent protein ffDronpa targeted to the nucleusDepositorArticleInsertffDronpa-H2B
UseTagsH2B targetting with a linker of 6AAExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pvimentin-ffDronpaF
Plasmid#214607PurposeExpresses reversibly switchable fluorescent protein ffDronpaF targeted to vimentinDepositorArticleInsertffDronpaF-vimentin
UseTagstarget to vimentin (human)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pffDronpa2F-peroxisome
Plasmid#214608PurposeExpresses reversibly switchable fluorescent protein ffDronpa2F targeted to peroxisomeDepositorArticleInsertffDronpa2F-peroxisome
UseTagsperoxisome targeting sequence (PTS)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACS125
Plasmid#204593PurposeGST-lamin A C522A (389-646)DepositorInserthuman lamin A C522A (389-646)
UseTagsGST - lamin A C522A (389-646)ExpressionBacterialMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACS38
Plasmid#204590PurposeGST-Progerin(506-664 delta 50)DepositorInserthuman progerin (506-664 delta 50)
UseTagsGST - progerin (506-664 delta 50)ExpressionBacterialMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-eIF4E-TwStrep
Plasmid#208638Purposefor E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)DepositorInserthuman eIF4E (ENST00000450253) (EIF4E Human)
UseTags6xHis and Twin StrepExpressionBacterialMutationPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-mEGFP(A206K)-TwStrep
Plasmid#208622Purposefor E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) cloneDepositorInsertmEGFP(A206K)
UseTags6xHis and Twin StrepExpressionBacterialMutation(compared with pEGFP-C1, protein level) A206KPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YDHFR_R127ATS
Plasmid#191312PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y DHFR/R127A TYMSPromoterT7 (folA), Tet (thyA)Available sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.18_TagBFP
Plasmid#205533PurposeExpress TagBFPDepositorInsertTagBFP
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP_sgRNA
Plasmid#190128PurposeLevel 0 MoClo plasmid for Golden Gate cloning of sgRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP_dCas9
Plasmid#190131PurposeLevel 0 MoClo plasmid containing dCas9DepositorInsertdCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
sgRNA5_8xCTS-ECFP-pA
Plasmid#200247PurposeMammalian transfections; 8xCTS-ECFP reporter construct 5DepositorInsertsgRNA5_8xCTS
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
sgRNA3_8xCTS-ECFP-pA
Plasmid#200245PurposeMammalian transfections; 8xCTS-ECFP reporter construct 3DepositorInsertsgRNA3_8xCTS
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
sgRNA2_8xCTS-ECFP-pA
Plasmid#200244PurposeMammalian transfections; 8xCTS-ECFP reporter construct 2DepositorInsertsgRNA2_8xCTS
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
U6-TM2Emp-6T_iBlue-ModFlanks
Plasmid#200236PurposeBackbone for cloning longer RNA triggers that activate iSBH-sgRNAsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_D27NDHFR_Q33STS
Plasmid#191289PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationD27N DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FG121VDHFR_Q33STS
Plasmid#191292PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationM42F/G121V DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YDHFR_R166QTS
Plasmid#191311PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y DHFR/R166Q TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YDHFR_WTTS
Plasmid#191310PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y DHFR/WT TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YDHFR_Q33STS
Plasmid#191313PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y DHFR/Q33S TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only