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Plasmid#14610DepositorInsertcyclin T1 (1-280) (all 200) (CCNT1 Human)
UseTagsmycExpressionMammalianMutationC198A, C200A, H202A, C205A. Active in 3T3 cells.PromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-HA-MEK1 C121S
Plasmid#53164PurposeExpresses GST-HA tagged human MEK1 C121S from bacterial expression vectorDepositorInsertMEK1 (MAP2K1 Human)
UseTagsGST and HAExpressionBacterialMutationC121SPromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAN19-3xFLAG-TIR1-NOSt
Plasmid#108545PurposeCloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminatorDepositorInsertTRANSPORT INHIBITOR RESPONSE 1 fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
UseCloning vectorTags3xFLAGExpressionMutationPromoterAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cntn6-AP-His
Plasmid#71944PurposeExpresses the extracellular region of the Contactin 6 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertCntn6 (Cntn6 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pScalps_puro_mIL-2Rα RK
Plasmid#59920PurposeExpresses mouse interleukin-2 receptor alpha chain mutatedDepositorInsertinterleukin 2 receptor alpha (Il2ra Mouse)
UseLentiviralTagsExpressionMutationchanged sequence of the intracellular (C-terminal…PromoterAvailable sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
RalA (G23V)( full )-pcw107-V5
Plasmid#64645Purpose(control) when used to produce lentivirus, express physiological levels of insertDepositorInsertRALA (RALA Human)
UseLentiviralTagsV5ExpressionMutationG23VPromoterPGKAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLAP-MIS12-KARD
Plasmid#114057Purposetransfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purificationDepositorInsertMIS12-KARD (BUB1B Human)
UseTagsYFP-TEV-S- Human LAPExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
RON6-bio
Plasmid#47785PurposeExpresses enzymatically monobiotinylated full-length RON6 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised RON6
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin replaced…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCSP1-mp53AS-6xMUT
Plasmid#20904DepositorInsertp53AS (Trp53 Mouse)
UseTagsExpressionMammalianMutationpoint mutation (Ser/Thr-Ala) of the 6 N-terminal …PromoterAvailable sinceApril 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
Anti-Synaptotagmin-10 [N269/73R]
Plasmid#114558PurposeMammalian Expression Plasmid of anti-Synaptotagmin-10 (Mouse). Derived from hybridoma N269/73.DepositorInsertanti-Synaptotagmin-10 (Mus musculus) recombinant mouse monoclonal antibody (Syt10 Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Laccase2 MCS-HIPK3 Exon
Plasmid#69888PurposeExpresses the human HIPK3 exon 2 circular RNA in DrosophilaDepositorInsertHIPK3 (HIPK3 Fly, Human)
UseTagsExpressionInsectMutationExpresses aa1-194 of HIPK3PromoterMetallothionein Promoter (pMT)Available sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-human LIC1 G domain
Plasmid#74598Purposeused for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389DepositorInserthuman dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)
UseTagsN-terminal GST and Strep IIExpressionBacterialMutationPromoterT7Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pD10AH840AhCas9 (GB1041)
Plasmid#68207PurposeProvides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid partDepositorInsertCas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removed; human codon optimis…PromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
RalA (G23V)( full )-pcw107
Plasmid#64644Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertRALA (RALA Human)
UseLentiviralTagsExpressionMutationG23VPromoterPGKAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-HA-MEK1 CBM
Plasmid#53163PurposeExpresses GST-HA tagged human MEK1 M187AH188AM230AH239A from bacterial expression vectorDepositorInsertMEK1 (MAP2K1 Human)
UseTagsGST and HAExpressionBacterialMutationM187A/H188A/M230A/H239APromoterAvailable sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-HA-MEK5 CBM-E
Plasmid#53168PurposeExpresses GST-HA tagged human MEK5 L280AH281AM323AQ332A from bacterial expression vectorDepositorInsertMEK5 (MAP2K5 Human)
UseTagsGST and HAExpressionBacterialMutationL280A/H281A/M323A/Q332APromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag4A-MMP8 (G104R)
Plasmid#29548DepositorInsertMetalloproteinase protein 8 (MMP8 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationG104RPromoterAvailable sinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSuper DGK zeta human
Plasmid#224575PurposeTo downregulate the expression of human DGK zeta in human cellsDepositorInsertsh RNAi Diacylglycerol kinase zeta human (DGKZ Human)
UseRNAiTagsExpressionMutationPromoterH1Available sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuper Retro GFP DGK alfa mouse
Plasmid#224576PurposeNegative Control for downregulation of the expression of human DGK alfa in human cellsDepositorInsertsh RNAi Diacylglycerol kinase alfa mouse (Dgka Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterH1Available sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuper Retro GFP DGK zeta human
Plasmid#224579PurposeTo stably downregulate the expression of human DGK zeta in human cellsDepositorInsertsh RNAi Diacylglycerol kinase zeta human (GTPBP6 Human)
UseRNAi and RetroviralTagsExpressionMutationPromoterH1Available sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only