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Plasmid#216171PurposeExpress the gRNA targeting the PRDM1-binding site in the human ZEB2 locus for the CRISPRd experimentDepositorInsertsgRNA- PRDM1.bs- ZEB2.locus (PRDM1 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
CB6-GFP-TAOK1 (K57A)
Plasmid#197109PurposeExpression of kinase-deficient GFP-tagged TAOK1 (K57A) / PSK2 (K57A) in mammalian cellsDepositorInsertTAOK1 (K57A) (TAOK1 Human)
UseTagsGFPExpressionMammalianMutationChanged K 57 to APromoterCMVAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBABE-neo-hTERT
Plasmid#1774PurposeRetroviral expression of hTERT. Used to create immortalized cell lines.DepositorInserthTERT (TERT Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pU6-SAG1-DHFR
Plasmid#80322PurposeEncodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamineDepositorInsertSAG1 sgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8541 PB-PGK-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-pA
Plasmid#140222PurposeConstitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPRDepositorInsertzipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
UseTagsmCherryExpressionMammalianMutationD832APromoterPGKAvailable sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-DHFR
Plasmid#80329PurposeAllows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamineDepositorInsertDHFR-TSc3
UseCRISPRTagsExpressionMutationPromoterDHFR-TS 5' UTRAvailable sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAEMXT-eLACCO1.1
Plasmid#167946PurposeFluorescent biosensor for extracellular L-lactateDepositorInsertCD59-eLACCO1.1-GPI
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHis2-Arf1 (17-181)-Q71L
Plasmid#184808PurposeBacterial expression of Arf1 (aa17-181, Q71L mutation)DepositorInsertArf1 (ARF1 Human)
UseTagsHis6ExpressionBacterialMutationaa 17-181 only, Q71LPromoterT7Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR-sgUBA5
Plasmid#86132PurposeLentiviral vector expressing Cas9 and an sgRNA targeting UBA5DepositorInsertsgRNA targeting UBA5 (UBA5 )
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMay 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAP2
Plasmid#73211Purposeexpress Assembly-activating protein (AAP) of adeno-associated virus serotype 2 (AAV2)DepositorInsertAAP2
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
SRSF8_RRM_pGEX
Plasmid#135119PurposeRecombinant expression of SRSF8 with dual-affinity tagDepositorInsertSRSF8 (SRSF8 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-Decoy
Plasmid#80324PurposeEncodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondiiDepositorInsertDecoy sgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAEMXT-deLACCO
Plasmid#167947PurposeFluorescent biosensor control for extracellular L-lactateDepositorInsertCD59-deLACCO-GPI
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
SRSF9_2xRRM_pGEX
Plasmid#135120PurposeRecombinant expression of SRSF9 with dual-affinity tagDepositorInsertSRSF9 (SRSF9 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
HNRNPCL1_RRM_pGEX
Plasmid#135116PurposeRecombinant expression of HNRNPCL1 with dual-affinity tagDepositorInsertHNRNPCL1 (HNRNPCL1 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceNov. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXs2-RAC1
Plasmid#86138PurposeRetrolviral vector expressing RAC1-IRES-Bsd-p2A-RFPDepositorInsertRAC1 (RAC1 Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
RBM15B_3xRRM_pGEX
Plasmid#135125PurposeRecombinant expression of RBM15B with dual-affinity tagDepositorInsertRBM15B (RBM15B Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2-TTF-1-gRNA
Plasmid#101857PurposeIntroducing a gRNA targeting human TTF-1 (NKX2-1) into cellsDepositorInsertgRNA targeting TTF-1 (NKX2-1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLN410 (FuGW-S(E2F1)p-ECFP sponge control)
Plasmid#105182PurposeModule 2 control - synthetic promoter E2F1 drives ECFP only (no miR1 binding sites)DepositorInsertS(E2F1)p-ECFP (Cerulean) sponge control
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs-RAP2A-GFP
Plasmid#86124PurposeRetroviral vector expressing RAP2A-GFP-IRES-BsdDepositorInsertRAP2A (RAP2A Human)
UseRetroviralTags3xFLAG and GFPExpressionMammalianMutationPromoterLTRAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only