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We narrowed to 13,599 results for: pET

Showing: 8241 - 8260 of 13599 results

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pLenti-EF1a-SPdCas9-EGFP-2A-Blast

Plasmid

#71215

Purpose

dCas9 fused to EGFP

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-EGFP

Use

Lentiviral

Tags

dSpCas9-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-IFITM3g1 (BB15)

Plasmid

#139460

Purpose

Lentiviral vector with gRNA targeting IFITM3; includes puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

IFITM3-targeting sgRNA inserted; resistance gene: puroR (IFITM3 Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a / U6

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNT.2#2/Cre

Plasmid

#173661

Purpose

Expresses a NT.2-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

Non-targeting gRNA

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-JAK1K908E-VSV

Plasmid

#139357

Purpose

Expression of kinase-dead JAK1-K908E protein

Depositor

Sandra Pellegrini

Article

Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

Insert

JAK1 cDNA with 300nt of 5' UTR (JAK1 Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

K908E

Promoter

CMV

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-irf3-shrna5

Plasmid

#127649

Purpose

Knock-down of human IRF3

Depositor

Nicolas Manel

Article

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

IRF3 shRNA (IRF3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-EGFP-T2A-Bsd

Plasmid

#193198

Purpose

Lentiviral vector expressing EGFP (driven by EFS promoter) and a blasticidin resistance marker

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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2xNLS-NLP-cMyc LbaCas12a

Plasmid

#182123

Purpose

pET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

2xNLS-NLP-cMyc LbaCas12a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pmiRGLO-ADGRL1-201

Plasmid

#117336

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR ADGRL1-201

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Murine PGK-1

Available since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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P119 C-Check

Plasmid

#66817

Purpose

Dual-fluorescent surrogate reporter vector for in vitro functional assay of programable DNA nuclease and enrichment of genetically edited cells

Depositor

Yonglun Luo

Article

Zhou et al Cell Mol Life Sci. 2016 Jan 11.

Insert

truncated Enhanced Green Fluorescent Protein and asRED

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK and CMV

Available since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-JAK1P1084A-VSV

Plasmid

#139358

Purpose

Expression of the catalytically impaired JAK1-P1084A mutant (corresponding to TYK2-P1104A)

Depositor

Sandra Pellegrini

Article

Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

Insert

JAK1 cDNA with 300nt of 5' UTR (JAK1 Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

P1084A

Promoter

CMV

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCALNL-Kir2.1

Plasmid

#125578

Purpose

Cre-dependent expression of Kir2.1 (FLAG-tagged) in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

Kir2.1 (Kcnj2 Mouse)

Use

Cre/Lox

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-BaeI-Pgk-Cre

Plasmid

#173663

Purpose

Vector with BaeI site to allow cloning of custom sgRNA into vector containing Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

BaeI site

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pJS647

Plasmid

#183176

Purpose

PYR1 mutagenesis plasmid compatible with pJS646 Y2H vector

Depositor

Timothy Whitehead

Article

Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

Insert

PYR1 (PYR1 Mustard Weed)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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His-DNPH1

Plasmid

#203224

Purpose

For protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

Depositor

Vern Schramm

Article

Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

Insert

DNPH1 (DNPH1 Human)

Use

Tags

His tag (with TEV cleavage site)

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pTAL_RFP

Plasmid

#43859

Depositor

Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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hEBI3 pEF6-V5 (MJS_002)

Plasmid

#72490

Purpose

Expression of human EBI3 in mammalian cells (V5 tag)

Depositor

Matthew Sweet

Article

Ramnath et al Immunol Cell Biol. 2015 Oct;93(9):771-9.

Insert

EBI3 (EBI3 Human)

Use

Tags

His/V5

Expression

Mammalian

Mutation

Promoter

EF-1 alpha

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Erk2

Plasmid

#40777

Purpose

Expression of rat Erk2 in mammalian cells

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2014 May 22;21(5):628-35. doi

Insert

Erk2 (Mapk1 Rattus norvegicus)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Starting Met removed

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
pTAL_GFP

Plasmid

#43858

Depositor

Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2012 Dec 12. pii: S1934-

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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hPlekhm1-dsRedM

Plasmid

#73592

Purpose

Express human Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

Insert

Plekhm1 (PLEKHM1 Human)

Use

Tags

dsRed

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
pmiRGLO-SERINC2-202

Plasmid

#117364

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR SERINC2-202

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Murine PGK-1

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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