-
Plasmid#155135PurposeExpresses N-terminally GFP- and HA-tagged E. coli GlpG from pGEX 4P-1DepositorInsertGlpG (glpG Escherichia coli)
UseTagsGST and HAExpressionBacterialMutationPromotertacAvailable sinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET-triple mutation-pa-GCaMP6f
Plasmid#67570Purposebacterial expression of a photoactivatable fluorescent calcium reporterDepositorInsertpa-GCaMP6f
UseTagsHIS-tagExpressionBacterialMutationV115H, L64F, T65SPromoterT7Available sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3E-FRB(PLF)-HA no-pA
Plasmid#82597Purpose3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (K2095P, T2098L, W2101F) without pA for rapamycin/rapalog induced dimerizationDepositorInsertFRB-HA (no pA)
UseTagsExpressionMutationK2095P, T2098L, W2101FPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_RBFOX2-Halo
Plasmid#175304PurposeProduction of HaloTag fusion proteinDepositorInsertRBFOX2 (RBFOX2 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Avr2-GFP pZK538
Plasmid#118011Purposeexpresses Avr2-GFP (without signal peptide) and mCherry-HDEL. Used for visualization of cell-to-cell movement of Avr2. Avr2 can be replaced with gene of interest via HiFi DNA assembly cloning (NEB) .DepositorArticleInsertsdspAvr2
mCherry
UseTagsEGFP and HDELExpressionBacterial and PlantMutationPromoter35SAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRAN334
Plasmid#120814PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt)DepositorInsertcyan fluorescent protein, codon-optimized for low GC organisms
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
ZP62 Cystathionine beta-synthase
Plasmid#16880DepositorInsertcystathionine beta-synthase (cbsb Zebrafish)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136462
Plasmid#149533PurposeBacterial expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTags6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEVExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMBP_hBrg1 (J1 antibody)
Plasmid#26109DepositorInsertBrg1 (SMARCA4 Human)
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
sspa-GCaMP6s
Plasmid#67559Purposeshort photoactivatable fluorescent calcium reporterDepositorInsertspa-GCaMP6s
UseTagsExpressionMammalianMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…PromoterAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEntra1a-mTurquoise2-3xrGBD
Plasmid#176137PurposeVisualization of active RhoDepositorInsertrhotekin (Rtkn Mouse)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-C-8His-Flag
Plasmid#157678PurposeC-terminal tagging vectorDepositorInsert8His-Flag
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046932
Plasmid#145750PurposeBacterial Expression plasmid for SARS-CoV-2 nsp9DepositorInsertSARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
groSL-GFP
Plasmid#109389PurposeGFP cassette under the control of the groSL sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertpgroSL-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
htpG2-GFP
Plasmid#109388PurposeGFP cassette under the control of the htpG2 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertphtpG2-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTX1-EV71-SL2
Plasmid#126043PurposeIn-vitro-transcription of Enterovirus 71 5UTR-SL2 RNA in NMR tube for "Systems NMR" analysisDepositorInsertEnterovirus 71 5'UTR SL2
UseTagsExpressionMutationPromoterAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-(RTRG)6
Plasmid#69849PurposeThe RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeatsDepositorInsert6 RTRG repeats
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
Human NCKX2-AS-long (pcDNA3.1+)
Plasmid#75201PurposeMammalian expression of SLC24A2DepositorInsertSLC24A2 (SLC24A2 Human)
UseTagsExpressionMammalianMutationalternatively spliced exon presentPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-mBcl11b-MAR4
Plasmid#178188PurposeLucifease reporter driven by MAR4 regulatory sequences of mouse Bcl11bDepositorInsertBcl11b MAR4
UseLuciferaseTagsExpressionMammalianMutationPromoterSV40 promoterAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4095421
Plasmid#149524PurposeBacterial expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationd1-745 d1063-1945PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits