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TRAC_DARIC(2-3)_CD22-BBz_AAV6_Donor

Plasmid

#211906

Purpose

Vector for AAV6 donor production. Targeted CD22-BBz DARIC transgene integration to the TRAC locus via homology-directed repair.

Depositor

Yannick Doyon

Article

Levesque et al bioRxiv 2023.09.14.557485

Insert

DARIC-CD22-BBz

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAV125

Plasmid

#63191

Purpose

pRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pK1E-RNAP

Plasmid

#48144

Purpose

Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylA

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

rna polymerase K1E

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-NLS-SNAP

Plasmid

#174879

Purpose

PiggyBac transposon vector for expression of NLS-SNAP

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

NLS-SNAP

Use

Piggybac vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-LGC-53

Plasmid

#86399

Purpose

Mammalian expression of codon-optimized dopamine receptor LGC-53

Depositor

Steven Mennerick

Article

Kress et al J Neurosci. 2014 Aug 27;34(35):11792-802

Insert

LGC-53 (lgc-53 Nematode, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Codon optimized for mammalian expression; Signal …

Promoter

CMV

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pHS1184

Plasmid

#205965

Purpose

NZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

DinG HNH

Use

Tags

14xHis-TwinStrep-bdSUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSb_init containing anti SARS-CoV-2_RBD sybody Sb#68

Plasmid

#153527

Purpose

Expression of the anti SARS-CoV-2_RBD sybody Sb#68

Depositor

Markus Seeger

Article

Walter et al BioRxiv 2020.04.16.045419

Insert

Sb#68

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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Human NANOS3-P2A-mCherry knock-in targeting construct

Plasmid

#59721

Purpose

Gene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.

Depositor

Jacob Hanna

Article

Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

Insert

P2A mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pET41a iGluh

Plasmid

#119830

Purpose

Production of recombinant iGluSnFR E25A high affinity variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluh

Use

Tags

GST

Expression

Bacterial

Mutation

E25A

Promoter

T7

Available since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pGex-Cav1

Plasmid

#33364

Depositor

Ambra Pozzi

Article

Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

Insert

Cav1 (CAV1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA3-myc3-FBW5

Plasmid

#19905

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

F-box and WD repeat domain containing 5 (FBXW5 Human)

Use

Tags

myc3

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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SspB-iRFP-DHPH-TIAM1

Plasmid

#176118

Purpose

Heterodimerization with iLID, Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-GLT1a

Plasmid

#162515

Purpose

Rat GLT-1a cDNA N-terminally tagged with EGFP

Depositor

Josef Kittler

Article

Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

Insert

GLT1a (Slc1a2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSB3K3 containing mRFP1

Plasmid

#118083

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, mRFP1

Use

Escherichia coli

Tags

Expression

Mutation

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-SspB-HaloTag-TIAM1-DHPH

Plasmid

#176127

Purpose

Heterodimerization with iLID, Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG TORC3

Plasmid

#22976

Depositor

Marc Montminy

Article

Screaton et al Cell. 2004 Oct 1. 119(1):61-74.

Insert

TORC3 (CRTC3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pET28a-Beclin1 CC

Plasmid

#24403

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains the CC domain (aa 151-242)

Promoter

Available since

April 1, 2010

Availability

Academic Institutions and Nonprofits only
pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-dSaCas9-2xNLS

Plasmid

#107319

Purpose

Expresses SpCas9 fused to dSaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 is fused to nuclease dead SaCas9 (D10A, N5…

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-AC3CtermGenomic-mCherry

Plasmid

#105250

Purpose

mouse mCherry (with STOP and extra-sequence) with homology arms for Adcy3

Depositor

Geraldine Seydoux

Article

Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

Insert

mCherry (with STOP and extra-sequence) with homology arms for mouse Adcy3 (Adcy3 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pSW609 - N001

Plasmid

#115993

Purpose

intermediate vector for GreenGate cloning method, contains modules from A-F

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

H-A adapter:pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR

Use

Unspecified

Tags

HDEL and SP(ER)

Expression

Mutation

Promoter

Available since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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