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Plasmid#64077PurposeExpression of human 5'-methylthioadenosine phosphorylaseDepositorInsert5'-methylthioadenosine phosphorylase (MTAP Synthetic, Human)
UseTagsHistidine tag and TEVExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
TR-Csy4-H29A
Plasmid#80602PurposeExpresses Csy4 with an inactivating H29A mutation from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertCsy4-H29A
UseAAVTagsExpressionMammalianMutationHis29 mutated to AlaPromoterCBAAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTF-ahpC-rfp
Plasmid#153035PurposeFor CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB6638
Plasmid#106165PurposeEasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6679 into Yarrowia lipolytica chromosomal location IntE_4, amp resistanceDepositorInsertunknown
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
TJP1_PDZ_1
Plasmid#103909PurposeProtein expression and purification of ZO1 PDZ1 domainDepositorInsertTJP1 (TJP1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK-Myc-Smurf1 delHECT
Plasmid#13677DepositorInsertSmurf1 deltaHect (SMURF1 Human)
UseTagsMycExpressionMammalianMutationSmurf1 without HECT domain (deleted amino acids 3…PromoterAvailable sinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Golgi-7
Plasmid#57382PurposeLocalization: Golgi Complex, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertGolgi (B4GALT1 Human)
UseTagsmEos2ExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pWZL blast HA goosecoid
Plasmid#14096DepositorInsertgoosecoid (Gsc Mouse)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
TagRFP-T-Rabenosyn-5
Plasmid#37537DepositorInsertRabenosyn5 (RBSN Human)
UseTagsTagRFP-TExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ macroH2A1.2-GFP-HA
Plasmid#30515DepositorInsertmacroH2A1.2-C (Macroh2a1 Mouse)
UseXenopus/zebrafish expression.TagseGFP and triple HAExpressionMammalianMutationNO STOPPromoterAvailable sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCOLA kiwi sensor sfGFP
Plasmid#107368PurposeExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified kiwi DNADepositorInsertkiwi-detecting toehold + sfGFP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lepr peak reporter-eGFP
Plasmid#196584PurposeCis-regulatory region of mouse Lepr gene driven a eGFP reporter with hGFP driven rtTA3DepositorInserteGFP
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterSV40Available sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
P2A-mCherry-EhT backbone
Plasmid#215549PurposeAn empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of hygromycin-NLS-tdTomatoDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV0758
Plasmid#133511PurposeExpression of 3GFP tagged RasAct probe to monitor active Ras1DepositorInsertpLys3(BstZ17I)-p(pak1)-RasAct(3xbyr2RBD)-3GFP-kanMX-terminator(ScADH1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0757
Plasmid#133509PurposeExpression of 3GFP tagged CRIB domain to monitor active Cdc42DepositorInsertpHis5(StuI)-p(pak1)-CRIB(gic2aa2-181)-3GFP-terminator(ScADH1)-kanMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0783
Plasmid#133515PurposeExpression of mCherry tagged LifeAct probe to monitor actin cytoskeletonDepositorInsertpAde6(PmeI)-p(act1)-LifeAct-mCherry-terminator(ScADH1)-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRU61
Plasmid#167692PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::asCpf1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUAST cyto roGFP2-Orp1
Plasmid#64996Purposefor expression of cytosolic roGFP2-Orp1 with the Gal4 expression system in drosophilaDepositorInsertOrp1 (HYR1 Budding Yeast)
UseTagsroGFP2ExpressionInsectMutationPromoterAvailable sinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAB082a
Plasmid#79233PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-Cry1Ac-d123
UseTagsExpressionBacterialMutationPromoterPpro1Available sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBacPAK p110a WT
Plasmid#55722PurposeHis3 GST tagged WT p110 alphaDepositorInsertp110 alpha (Pik3ca Mouse)
UseTagsHis3 GSTExpressionMutationPromoterpolyhedrin promoterAvailable sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only