Showing: 8201 - 8220 of 20568 results
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Plasmid#99694PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAVDepositorArticleInsertdCas9 and gRNA targeting Actc1 (gRNA: GGTATATAAGGGGTTTTAAG) (Actc1 Synthetic, Mouse)
UseAAV and CRISPRTagsVPR miniExpressionMutationdead Cas9PromoterAvailabilityAcademic Institutions and Nonprofits only -
AAV-nEF-dCas9
Plasmid#106430PurposeExpresses SpdCas9 from the nEF promoter in AAV backboneDepositorInsertnEF-dCas9
UseAAV and CRISPRTagsHA-NLS and NLSExpressionMammalianMutationPromoternEFAvailabilityAcademic Institutions and Nonprofits only -
AAV-CMVc-Cas9
Plasmid#106431PurposeExpresses SpCas9 from the CMVc promoter in AAV backboneDepositorInsertCas9
UseAAV and CRISPRTagsHA-NLS and NLSExpressionMammalianMutationPromoterCMVcAvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpRY-P2A-EGFP (RTW4830)
Plasmid#139989PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)
Plasmid#139996PurposeCMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…PromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)
Plasmid#139997PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)
Plasmid#140003PurposeCMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…PromoterCMV and T7AvailabilityAcademic Institutions and Nonprofits only -
pJR85
Plasmid#140095PurposeLentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.DepositorInsertsgRNA with cs1 in stem loop 2 of the constant region
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBA900
Plasmid#122237PurposeLentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs2 incorporated at the 3' end of the sgRNA constant region.DepositorInsertsgRNA with cs2 at the 3' end of the constant region
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBA904
Plasmid#122238PurposeLentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.DepositorInsertsgRNA with cs1 in stem loop 2 of the constant region
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJEP311-pAAV-EFS-SaCas9-P2A-HAFLAGHA-KASH-pA
Plasmid#113688PurposeSaCas9 driven by EFS. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.DepositorInsertSaCas9 (NEWENTRY )
UseAAV and CRISPRTagsFlag, HAx2, KASH, and NLSExpressionMutationPromoterCytomegalo Virus(CMV)AvailabilityAcademic Institutions and Nonprofits only -
pUDR295
Plasmid#113874Purposeexpression of Cas9 programming sgRNA2 and sgRNA1 targetting GAL2 and HXT4-1-5/ HXT3-6-7 respectivelyDepositorInsertsgRNA2 GAL2 sgRNA1-HXT4-1-5;HXT3-6-7
UseCRISPRTagsExpressionYeastMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTE4497
Plasmid#80339Purposemammalian expression FnCpf1 nuclease and Fn crRNADepositorInsertsFn crRNA
FnCpf1
UseCRISPRTags3xHA and NLSExpressionMammalianMutationPromoterCMV and human U6AvailabilityAcademic Institutions and Nonprofits only -
CCL2 gRNA (BRDN0001147233)
Plasmid#77865Purpose3rd generation lentiviral gRNA plasmid targeting human CCL2DepositorInsertCCL2 gRNA (CCL2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6AvailabilityAcademic Institutions and Nonprofits only -
CCL2 gRNA (BRDN0001145563)
Plasmid#77866Purpose3rd generation lentiviral gRNA plasmid targeting human CCL2DepositorInsertCCL2 gRNA (CCL2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6AvailabilityAcademic Institutions and Nonprofits only -
CCL2 gRNA (BRDN0001148290)
Plasmid#77867Purpose3rd generation lentiviral gRNA plasmid targeting human CCL2DepositorInsertCCL2 gRNA (CCL2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6AvailabilityAcademic Institutions and Nonprofits only -
CCL2 gRNA (BRDN0001147096)
Plasmid#77868Purpose3rd generation lentiviral gRNA plasmid targeting human CCL2DepositorInsertCCL2 gRNA (CCL2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6AvailabilityAcademic Institutions and Nonprofits only -
JAK2 gRNA (BRDN0001146541)
Plasmid#75726Purpose3rd generation lentiviral gRNA plasmid targeting human JAK2DepositorInsertJAK2 (JAK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6AvailabilityAcademic Institutions and Nonprofits only -
JAK2 gRNA (BRDN0001147052)
Plasmid#75727Purpose3rd generation lentiviral gRNA plasmid targeting human JAK2DepositorInsertJAK2 (JAK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6AvailabilityAcademic Institutions and Nonprofits only -
JAK2 gRNA (BRDN0001149125)
Plasmid#75728Purpose3rd generation lentiviral gRNA plasmid targeting human JAK2DepositorInsertJAK2 (JAK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6AvailabilityAcademic Institutions and Nonprofits only
Showing: 8201 - 8220 of 20568 results