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Plasmid#82718PurposeExpresses constitutively active human MKK6. Multiple cloning site 2 is empty.DepositorInsertMKK6 (MAP2K6 Human)
UseTagsExpressionBacterialMutationSer207 to Glu, Thr211 to GluPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
stabilized HP35 tension sensor module
Plasmid#101251PurposeExpresses the stabilized HP35-based tension sensor module. The biosensor allows force measurements at 9-11 pN.DepositorInsertYPet(short)-HP35st-mCherry
UseRetroviralTagsHis-tag and cysteineExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
F40 tension sensor
Plasmid#101252PurposeExpresses the F40-based tension sensor module. The biosensor allows force measurements at 1-6 pN.DepositorInsertYPet(short)-F40-mCherry
UseRetroviralTagsHis-tag and cysteineExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
RGG-mCherry-RGG-eDHFR
Plasmid#164648PurposeTwo RGG domains tagged with mCherry and eDHFRDepositorInsertRGG
UseCre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgHK2_2
Plasmid#163461Purposelentiviral vector expressing Cas9 and an sgRNA targeting HK2DepositorInsertsgRNA 2 targeting HK2 (HK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pProEX-Af2Sir2
Plasmid#8380DepositorUseTagsHisExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLKO-shcIAP1-B
Plasmid#44131DepositorInsertc-IAP1 (BIRC2 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
PET11a-Af2sir2
Plasmid#8383DepositorUseTagsExpressionBacterialMutationPET11a-Af2Sir2D103NPromoterAvailable sinceDec. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMX-Flag-WIP 460 deletion
Plasmid#11773DepositorInsertWIP aa1-460 (WIPF1 Human)
UseRetroviralTagsflagExpressionMammalianMutationaa461-503 deletedPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEY80
Plasmid#191075Purposeflp-18(AVA = 4.2-1k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertflp-18(AVA = 4.2-1k) (flp-18 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-dora[B]_rescue_construct
Plasmid#190638PurposePuromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTags3xHAExpressionInsectMutationChanged threonine 295 to methioninePromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-dora[A]_rescue_construct
Plasmid#190640PurposePuromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cellsDepositorInsertDorado (CG34401 Fly)
UseTags3xHAExpressionInsectMutationTruncated to contain amino acids 1-945PromoterActin-5cAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pLentiPGK Puro DEST ERKKTRClover
Plasmid#90227PurposeLentiviral vector to express ERK KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertERK Kinase Translocation Reporter
UseLentiviralTagsmCloverExpressionMammalianMutationPromoterPGKAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Mito-SBFP2
Plasmid#187964PurposeMammalian cell expression of a fluorescent SBFP2-tagged mitochondrial markerDepositorInsertMito (COX8A Human)
UseTagsSBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-CCO-IRES-Puromycin
Plasmid#134861PurposeExpresses EGFP-tagged inner mitochondrial membrane markerDepositorInsertcytochrome c oxidase subunit VIII (COX8A Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST JNKKTRmCerulean3
Plasmid#90232PurposeLentiviral vector to express JNK KTR mCerulean3 under PGK promoter (With Blasticidin Resistance)DepositorInsertJNK Kinase Translocation Reporter
UseLentiviralTagsmCerulean3ExpressionMammalianMutationPromoterPGKAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Myc-mMEKK2
Plasmid#44158DepositorInsertMekk2 (Map3k2 Mouse)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLJC6-GPT2-3xFLAG
Plasmid#163449Purposelentiviral overexpression vector for human GPT2DepositorInsertGPT2 (GPT2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationSee: commentsPromoterUbcAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJC2-GPT2-3xFLAG
Plasmid#163447Purposelentiviral overexpression vector for human GPT2DepositorInsertGPT2 (GPT2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationSee: commentsPromoterCMVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only