Addgene

We narrowed to 24,707 results for: sta

Showing: 8181 - 8200 of 24707 results

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pEF.DEST51-mVenus

Plasmid

#154899

Purpose

Expresses a fluorescent protein, mVenus

Depositor

Johanna Ivaska

Article

Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

Insert

mVenus

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF-1α

Available since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMP4518

Plasmid

#107778

Purpose

Expression of EYFP

Depositor

Herman Spaink

Article

Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

pLacZ-EYFP in pBBR1-mcs5

Use

Tags

pLacZ-EYFP

Expression

Bacterial

Mutation

Promoter

pLacZ

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pTorPE-jREX-GECO1

Plasmid

#113941

Purpose

Bacterial expression of red fluorescent Ca2+ sensor jREX-GECO1

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

jREX-GECO1

Use

Tags

6xHis and Modified TorA MNNNDLFQASRRRFLAQLG[G to …

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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TJP1_PDZ_2

Plasmid

#103910

Purpose

Protein expression and purification of ZO1 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

TJP1 (TJP1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1 BSMT1

Plasmid

#106287

Purpose

Expresses BSMT1 in Ecoli off a T7 promoter

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

BSMT1

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (TBE 1/2 - wt)

Plasmid

#16595

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (TBE 1/2 wt) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only
pCLX-M38s/TOP-dGFP

Plasmid

#114295

Purpose

Expression of destabilized GFP under the control of the M38-TOP canonical Wnt signaling responsive promoter

Depositor

Patrick Salmon

Article

Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

Insert

dGFP

Use

Lentiviral

Tags

Expression

Mutation

destabilized GFP

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAV0752

Plasmid

#133486

Purpose

Expression in fission yeast from promoter pnmt41 , integrated at ura4 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(nmt41)-sfGFP-terminator(ScCYC1)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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RFP-KIF3A-HL

Plasmid

#51397

Purpose

expression of RFP-tagged amino acid 369-701 (headless) of KIF3A in mammalian cells

Depositor

Trina Schroer

Article

Brown et al Traffic. 2005 Dec;6(12):1114-24.

Insert

KIF3A (Kif3a Mouse)

Use

Tags

RFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only
pBMN-FKBP DD(L106P)-UbK0G75C-sfGFP

Plasmid

#172001

Purpose

Expression for LIBRON constructs

Depositor

Thomas Wandless

Article

Miyamae et al Cell Chem Biol. 2020 Dec 17;27(12):1573-

Insert

FKBP DD (L106P)-UbK0G75C

Use

Retroviral

Tags

sfGFP

Expression

Mutation

change all lysin residues of ubiquitin to Arg, G7…

Promoter

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pAB061i2

Plasmid

#79229

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-HA4 (Y87A); PlacIq lacI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ppro1; PlacIq

Available since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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ibpAB-GFP

Plasmid

#109390

Purpose

GFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pibpAB-GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 (+) Tacδ

Plasmid

#81177

Purpose

Mammalian expression of human Tacδ (GFFKR cytoplasmic tail)

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

Tacδ

Use

Tags

KLGFFKR

Expression

Mammalian

Mutation

CD25 amino acids 1 to 263 fused to KLGFFKR cytopl…

Promoter

CMV

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pKF-P14MM2AG5h

Plasmid

#128254

Purpose

Mammalian positive feedback synthetic gene circuit within a Flp-In expression vector.

Depositor

Gabor Balazsi

Article

Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

Insert

rtTA-Advanced::P2A::EGFP

Use

Synthetic Biology; Flp-in expression vector

Tags

Expression

Mammalian

Mutation

Promoter

tight TRE promoter

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-AAVS1_sgRNA

Plasmid

#183891

Purpose

pX459V2.0-HypaCas9 plasmid with sgRNA targeting the AAVS1 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

AAVS1 sgRNA spacer (AAVS1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pET12a-AL-09 FL

Plasmid

#47081

Depositor

Marina Ramirez-Alvarado

Article

McLaughlin et al Protein Sci. 2006 Jul;15(7):1710-22. Epu

Insert

AL-09 (IGKV3-20 Human)

Use

Tags

ompT

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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Dendra2-Vimentin-7

Plasmid

#57745

Purpose

Localization: Intermediate Filaments, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Vimentin (VIM Human)

Use

Tags

Dendra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pMOD_A0108

Plasmid

#91001

Purpose

Module A, Promoter: EC1.2, Gene: AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

EC1.2

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAV0753

Plasmid

#133487

Purpose

Expression in fission yeast from promoter pnmt81 , integrated at ura4 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(nmt81)-sfGFP-terminator(ScCYC1)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSIBR003

Plasmid

#177666

Purpose

SIBR Intron 3 (Int3) interrupts the CDS of FnCas12a

Depositor

John van der Oost

Article

Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

lacUV5

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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