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  4. pCR4-*mRFP

    Plasmid
    #47118
    Purpose
    Can be used to make an RNA probe for whole-mount in situ hybridization, for in-frame fusion proteins or as a subcloning step for mRFP. Note that the coding sequence for mRFP lacks the start codon.
    Depositor
    Stephen Ekker
    Article
    Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub
    Insert
    *mRFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    No start codon
    Promoter
    Lac
    Available since
    Oct. 16, 2013
    Availability
    Academic Institutions and Nonprofits only
    47118-plasmid-map-sequence-id-67205

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  19. pCI-Flag-NEDD4L∆WW1,2,4

    Plasmid
    #171137
    Purpose
    expresses NEDD4L with inactivating mutation in first, second and fourth WW domain, but intact third WW domain, in mammalian cells
    Depositor
    Wesley Sundquist
    Article
    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10
    Insert
    NEDD4L (NEDD4L Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed tryptophan 221 to alanine, tryptophan 453…
    Promoter
    Available since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    171137-plasmid-map-sequence-id-338242

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Showing: 8181 - 8200 of 13599 results