Addgene

We narrowed to 907 results for: mir sensor

Showing: 801 - 820 of 907 results

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pRSET FLII12Pglu-600u

Plasmid

#13563

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A Y12S-ECFP-D13R

Use

Tags

Citrine

Expression

Bacterial

Mutation

F16A ECFP insertion in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-3.2mDelta13

Plasmid

#13560

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A S112A Delta13

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

F16A, S112A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pRSET FLII275Pglu-4.6m

Plasmid

#13565

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A G275S-ECFP-K276R

Use

Tags

Citrine

Expression

Bacterial

Mutation

F16A, ECFP insert in mglB

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-2uDelta13

Plasmid

#13561

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16F D152A Delta13

Use

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

F16A, D152A in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pRSET FLII32Pglu-2.2m

Plasmid

#13566

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A P32S-ECFP-D33R

Use

Tags

6xHis and Citrine

Expression

Bacterial

Mutation

F16A, ECFP insert in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pRSET FLII282Pglu-4m

Plasmid

#13564

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A T282S-ECFP-N283R

Use

Tags

Citrine

Expression

Bacterial

Mutation

F16A, ECFP insert in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE intramol-1u

Plasmid

#13549

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIP-E intramol WT (gltI E. coli)

Use

Tags

EYFP

Expression

Bacterial

Mutation

ECFP insert in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE intramol-1m

Plasmid

#13551

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIP-E intramol A207W (gltI E. coli)

Use

Tags

EYFP

Expression

Bacterial

Mutation

A207W, ECFP insert in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE intramol-10u

Plasmid

#13550

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIP-E intramol A207R (gltI E. coli)

Use

Tags

EYFP

Expression

Bacterial

Mutation

A207R, ECFP insert in binding protein

Promoter

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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MKV937/nlp-1p::FlincG3

Plasmid

#140508

Purpose

This construct contains cGMP biosensor driven by nlp-1 promoter for expression in PHB sensory neuron in pSMdelta backbone.

Depositor

Miri VanHoven

Article

Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

Insert

FlincG3

Use

Tags

Expression

Worm

Mutation

Promoter

nlp-1

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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p.UTA.2.0 Empty

Plasmid

#82446

Purpose

[Edit] Dual Fluorescent endogenous miRNA sensor. To insert the complementary region at the 3'UTR of CFP

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p.UTA.3.0 Empty

Plasmid

#82447

Purpose

[Edit] Dual Fluorescent endogenous miRNA sensor. To insert the complementary region of the 3'UTR of RFP.

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Type

Empty backbone

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAW-EGFP-4xlet-7 (pAW-AS27)

Plasmid

#40834

Purpose

miRNA sensor used in Drosophila cell lines

Depositor

Phillip Zamore

Article

Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

Insert

Four target sites perfectly complementary to let-7

Use

Mirna reporter

Tags

EGFP

Expression

Insect

Mutation

Promoter

Actin5C

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
p.UTA.2.0-GL-2

Plasmid

#82440

Purpose

Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of an siRNA control targeting firefly luciferase encoded by the pGL2

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Insert

Luciferase

Use

Tags

Expression

Mutation

The complementary region for GL2 siRNA control wa…

Promoter

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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mg319

Plasmid

#73367

Purpose

unc-70 tension sensor

Depositor

Miriam Goodman

Article

Krieg et al Nat Cell Biol. 2014 Mar;16(3):224-33. do

Insert

unc-70::TSMod

Use

Tags

Expression

Mutation

Promoter

unc-70p

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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p.UTA.2.0-GL-2-3 perfect copies

Plasmid

#82441

Purpose

Dual Fluorescent endogenous miRNA sensor Control insert. Three Perfect copies of complementary region of a siRNA control targeting firefly luciferase encoded by the pGL2

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Insert

Luciferase

Use

Tags

Expression

Mutation

The complementary region for GL2 siRNA control wa…

Promoter

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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WDV

Plasmid

#226983

Purpose

Expresses the HUH endonuclease WDV, used as a no ligand control for DNA tension sensor experiments

Depositor

Wendy Gordon

Article

Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

Insert

WDV (repA )

Use

Tags

6xHis and SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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WDV-Echistatin

Plasmid

#226985

Purpose

Expresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensors

Depositor

Wendy Gordon

Article

Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

Inserts

WDV (repA )
Echistatin

Use

Tags

6xHis and SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-MBP-yATOM_mCherry

Plasmid

#209707

Purpose

Mammalian expression of y-ATOM biosensor for mCherry

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_mCherry

Use

Tags

MBP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_Ras

Plasmid

#209709

Purpose

Mammalian expression of y-ATOM biosensor for HRas

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_Ras

Use

Tags

MBP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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