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Addgene
Showing: 801 - 820 of 3692 results

  1. pT7-EGFP-C1-HsNot6_165-557_K

    Plasmid
    #146739
    Purpose
    Mammalian Expression of HsNot6_165-557
    Depositor
    Elisa Izaurralde
    Insert
    HsNot6_165-557 (CNOT6 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146739-plasmid-map-sequence-id-289185

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  2. pT7-EGFP-C1-HsDCP1a_131-582_L

    Plasmid
    #146819
    Purpose
    Mammalian Expression of HsDCP1a_131-582
    Depositor
    Elisa Izaurralde
    Insert
    HsDCP1a_131-582 (DCP1A Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146819-plasmid-map-sequence-id-289266

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  3. pT7-EGFP-C1-HsRCK-K135A_H

    Plasmid
    #146455
    Purpose
    Mammalian Expression of HsRck-K135A
    Depositor
    Elisa Izaurralde
    Insert
    HsRck-K135A (DDX6 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146455-plasmid-map-sequence-id-288900

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  5. Venus-Bik-cb5-pEGFP-C1

    Plasmid
    #177431
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) replaced with Cb5 targeting sequence. Swap made in original Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Bik MBR removed (after position 136) and replaced…
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177431-plasmid-map-sequence-id-348459

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  6. Venus-Bik-delMBR-pEGFP-C1

    Plasmid
    #177432
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) deleted (Bik(1-136)). Compare to full length Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Bik MBR removed (after position 136)
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177432-plasmid-map-sequence-id-348460

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  7. mCherry-7aa-cb5-pEGFP-C1

    Plasmid
    #177407
    Purpose
    To express mCherry fused to endoplasmic reticulum (ER) targeting sequence "Cb5". mCherry faces the cytosol.
    Depositor
    David Andrews
    Insert
    Cb5 tail anchor (Cyb5r4 Rat)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177407-plasmid-map-sequence-id-348154

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  8. Venus-BclXL-ActA-pEGFP-C1

    Plasmid
    #177410
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-ActA: Bcl-XL with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-XL-ActA, BclX-acta
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177410-plasmid-map-sequence-id-348171

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  9. mCerulean3-BclXL-ActA-pEGFP-C1

    Plasmid
    #177412
    Purpose
    To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-ActA: Bcl-XL with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-XL-ActA, BclX-acta
    Use
    Tags
    mCerulean3
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177412-plasmid-map-sequence-id-348192

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  10. mCerulean3-BclXL-Cb5-pEGFP-C1

    Plasmid
    #177414
    Purpose
    To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-Cb5: Bcl-XL with its membrane binding region swapped for Cb5 (ER targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-XL-Cb5, BclX-cb5
    Use
    Tags
    mCerulean3
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177414-plasmid-map-sequence-id-348199

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  11. Venus-BclXL-Cb5-pEGFP-C1

    Plasmid
    #177415
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-Cb5: Bcl-XL with its membrane binding region swapped for Cb5 (ER targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-XL-Cb5, BclX-cb5
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177415-plasmid-map-sequence-id-348201

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  12. mCerulean3-Bcl2-ActA-pEGFP-C1

    Plasmid
    #177418
    Purpose
    To express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-2-ActA, Bcl2-acta, Bcl-acta
    Use
    Tags
    mCerulean3
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177418-plasmid-map-sequence-id-348297

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  13. Venus-Bcl2-ActA-pEGFP-C1

    Plasmid
    #177419
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-ActA: Bcl-2 with its membrane binding region swapped for ActA (mitochondrial targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-2-ActA, Bcl2-acta, Bcl-acta
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177419-plasmid-map-sequence-id-348305

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  14. mCerulean3-Bcl2-cb5-pEGFP-C1

    Plasmid
    #177421
    Purpose
    Express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-2-Cb5, Bcl2-cb5, Bcl-cb5
    Use
    Tags
    mCerulean3
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177421-plasmid-map-sequence-id-348306

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  15. Venus-Bcl2-cb5-pEGFP-C1

    Plasmid
    #177422
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-2-Cb5: Bcl-2 with its membrane binding region swapped for Cb5 (ER targeting sequence).
    Depositor
    David Andrews
    Insert
    Bcl-2-Cb5, Bcl2-cb5, Bcl-cb5
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177422-plasmid-map-sequence-id-353685

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  16. Venus-Bik-L61G-pEGFP-C1

    Plasmid
    #177426
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with L61G mutation in BH3 domain to disrupt binding anti-apoptotic proteins. Mutation made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Leucine 61 replaced with glycine
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177426-plasmid-map-sequence-id-348449

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  17. Venus-Bik-GG153AA-pEGFP-C1

    Plasmid
    #177427
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with G153A,G154A mutations in membrane binding region. Mutations made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Glycines 153 and 154 mutated to alanine
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177427-plasmid-map-sequence-id-348452

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  18. Venus-Bik-G154STOP-pEGFP-C1

    Plasmid
    #177428
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, truncatated within the membrane binding region (1-154). Stop codon added to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Glycine 154 replaced with STOP codon
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177428-plasmid-map-sequence-id-348454

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  19. Venus-Bik-DD-pEGFP-C1

    Plasmid
    #177429
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, phosphomimetic mutant. T33D and S35D mutations made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Threonine 33 and Serine 35 replaced with aspartic…
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177429-plasmid-map-sequence-id-348456

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  20. Venus-Bik-AA-pEGFP-C1

    Plasmid
    #177430
    Purpose
    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with 2 sites of phosphorylation mutated to alanine. T33A and S35A mutations made to Addgene #166737.
    Depositor
    David Andrews
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Threonine 33 and Serine 35 replaced with alanine
    Promoter
    CMV
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177430-plasmid-map-sequence-id-348458

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Showing: 801 - 820 of 3692 results