Skip to main content
Addgene
Showing: 801 - 820 of 3847 results

  1. pVenusNfN

    Plasmid
    #128564
    Purpose
    Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.
    Depositor
    Oliver Vugrek
    Article
    Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11
    Insert
    Venus
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    128564-plasmid-map-sequence-id-321698

    Enlarge

  2. pVenusCfC

    Plasmid
    #128567
    Purpose
    Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.
    Depositor
    Oliver Vugrek
    Article
    Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11
    Insert
    Venus
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    128567-plasmid-map-sequence-id-321691

    Enlarge

  3. pVenusCfN

    Plasmid
    #128566
    Purpose
    Protein interaction screening tool with Gateway-compatible BiFC vectors comprising of a set of 4 vectors permiting N or C fusions of candidate genes to the N or C fragment of Split-Venus.
    Depositor
    Oliver Vugrek
    Article
    Lepur et al J Biomol Screen. 2016 Dec;21(10):1100-11
    Insert
    Venus
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    128566-plasmid-map-sequence-id-321694

    Enlarge

  5. pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher

    Plasmid
    #117656
    Purpose
    Bacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.
    Depositor
    Bo Huang
    Article
    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.
    Inserts
    sfCherry2(1-10)
    sfCherry2(11)-SpyCatcher002
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    117656-plasmid-map-sequence-id-226764

    Enlarge

  8. IBMc396

    Plasmid
    #161954
    Purpose
    Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHH
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    March 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    161954-plasmid-map-sequence-id-328230

    Enlarge

  14. pSLQ8541 PB-PGK-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-pA

    Plasmid
    #140222
    Purpose
    Constitutive PGK driven expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR
    Depositor
    Stanley Qi
    Article
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Insert
    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    PGK
    Available since
    May 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140222-plasmid-map-sequence-id-296741

    Enlarge

  16. PE_CO-Mini-C

    Plasmid
    #190337
    Purpose
    Expressing the C-terminal of PE_CO-Mini using Rma intein and Cas9 673-674 split site, and pegRNA for PCSK9 +1A insertion
    Depositor
    Rasmus Bak
    Article
    Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22
    Insert
    PE_CO-Mini-C, PCSK9 +1A insertion pegRNA
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    190337-plasmid-map-sequence-id-374556

    Enlarge

  17. dCas9-NLS-gs10-cGR2176

    Plasmid
    #188258
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split GR2 (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    cGR2176
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    188258-plasmid-map-sequence-id-377988

    Enlarge

  20. PE_CO-Mini-N

    Plasmid
    #190336
    Purpose
    Expressing the N-terminal of PE_CO-Mini using Rma intein and Cas9 673-674 split site, ngRNA and pegRNA for PCSK9 +1A insertion
    Depositor
    Rasmus Bak
    Article
    Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22
    Insert
    PE_CO-Mini-N, ngRNA and pegRNA for PCSK9 +1A insertion
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    190336-plasmid-map-sequence-id-374544

    Enlarge

Showing: 801 - 820 of 3847 results