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Plasmid#196666PurposeExpression of C-terminally GST-distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
UseTagsGST and TEV-cleavable di-strep tag IIExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-strep-mClover3
Plasmid#196668PurposeExpression of C-terminally distrep tag II-mClover3-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
UseTagsTEV-cleavable di-strep tag II and mClover3ExpressionBacterialMutationPromoteraraBADAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-ZNF746
Plasmid#207819PurposeN-terminal tagged ZNF746 for miniTurbo proximity experimentsDepositorInsertminiTurbo-V5-ZNF746 (ZNF746 Human)
UseLentiviralTagsminiTurbo-V5ExpressionMutationPromoterUBCAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-ZNF746-V5-miniTurbo
Plasmid#207820PurposeC-terminal tagged ZNF746 for miniTurbo proximity experimentsDepositorInsertZNF746-V5-miniTurbo (ZNF746 Human)
UseLentiviralTagsV5-miniTurboExpressionMutationPromoterUBCAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-ZNF746-C571A
Plasmid#207821PurposeN-terminal tagged ZNF746-C571A for miniTurbo proximity experimentsDepositorInsertminiTurbo-V5-ZNF746-C571A (ZNF746 Human)
UseLentiviralTagsminiTurbo-V5ExpressionMutationC571APromoterUBCAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cas9-ZNF746
Plasmid#207822PurposeDual expression of Cas9 and sgRNA targeting ZNF746DepositorInsertZNF746 sgRNA (ZNF746 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterEF-1a; U6Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTij_sg_mmu_Med1_N_terminal
Plasmid#197870PurposeCRISPR/Cas9/sgRNA plasmid for cutting Med1 N terminal and building mEmerald/Halo-MED1 knock-in mESCsDepositorInsertCRSP210 (Med1 Mouse)
UseTagsExpressionMammalianMutationPromoterU6Available sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA
Plasmid#184636PurposeExpresses the GABA membrane transporter Gat1 R69K mutant fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashionDepositorInsertGat1 (Slc6a1 Mouse)
UseAAVTagsExpressionMutationGat1 gene with Arginine 69 changed to Lysine, to …PromoterEF1aAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_MTOR_Nick_Exon-53_Dual_sgRNA
Plasmid#173211PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
UseCRISPR; Prime editing (pe3)TagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_RUNX1_+4_T_to_G_Dual_pegRNA
Plasmid#173212PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +4 T to G pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + RUNX1 +4 T to G pegRNA
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_RUNX1_+1_ATGins_Dual_pegRNA
Plasmid#173213PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +1 ATG insertion pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + RUNX1 +1 ATG insertion pegRNA
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c2X-ORF24-NTD-3L_A
Plasmid#138466PurposeExpresses MBP-tagged ORF24-NTD 3L_A in bacteriaDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsMBPExpressionBacterialMutationAmino acids 1-201; amino acids L73-75 mutated to …PromotertacAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 1-201-2xStrep
Plasmid#138442PurposeExpresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsStrepExpressionMammalianMutationAmino acids 1-201PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 1-226-2xStrep
Plasmid#138443PurposeExpresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsStrepExpressionMammalianMutationAmino acids 1-226PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-BcRF1 1-168-2xStrep
Plasmid#138447PurposeExpresses truncated BcRF1 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertBcRF1 (BcRF1 EBV (HHV-4))
UseTagsStrepExpressionMammalianMutationAmino acids 1-168PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-BcRF1 1-178-2xStrep
Plasmid#138448PurposeExpresses truncated BcRF1 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertBcRF1 (BcRF1 EBV (HHV-4))
UseTagsStrepExpressionMammalianMutationAmino acids 1-178PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-BcRF1 1-203-2xStrep
Plasmid#138449PurposeExpresses truncated BcRF1 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertBcRF1 (BcRF1 EBV (HHV-4))
UseTagsStrepExpressionMammalianMutationAmino acids 1-203PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-UL87 1-238-2xStrep
Plasmid#138450PurposeExpresses truncated UL87 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertUL87 (UL87 HCMV (HHV-5))
UseTagsStrepExpressionMammalianMutationAmino acids 1-238PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-UL87 1-248-2xStrep
Plasmid#138451PurposeExpresses truncated UL87 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertUL87 (UL87 HCMV (HHV-5))
UseTagsStrepExpressionMammalianMutationAmino acids 1-248PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-UL87 1-273-2xStrep
Plasmid#138452PurposeExpresses truncated UL87 with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertUL87 (UL87 HCMV (HHV-5))
UseTagsStrepExpressionMammalianMutationAmino acids 1-273PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only