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Plasmid#172831PurposeMammalian expression of a sgRNA targeting the exon 2 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_Camk2a sgRNA / hSpCas9
Plasmid#172839PurposeMammalian expression of a sgRNA targeting the intron 17 (last intron) of Camk2a (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 17 of Camk2a under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i5 sgRNA / hSpCas9
Plasmid#172829PurposeMammalian expression of a sgRNA targeting the intron 1 position 5 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i3 sgRNA / hSpCas9
Plasmid#172827PurposeMammalian expression of a sgRNA targeting the intron 1 position 3 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i2 sgRNA / hSpCas9
Plasmid#172826PurposeMammalian expression of a sgRNA targeting the intron 1 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xPP7-Qb model based
Plasmid#158203PurposeCassette of 10 different binding sites with high affinity to both PP7 and Qb phage coats protein, as computed by the ML model.DepositorInsert10 binding sites with high affinity to PP7 and QB coat proteins based on the machine-learning model
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT46_GalL_cCDA1-BE3
Plasmid#145039PurposeExpresses cCDA1-BE3 in yeast cellsDepositorInsertcCDA1-BE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
Drosophila Nedd2-like caspase (DRONC)
Plasmid#157778PurposeE. coli expression vector pET23 with C-term 6xHis tagDepositorInsertDrosophila Nedd2-like caspase (DRONC) (Dronc Fly)
UseTags6x HISExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT139_GalL_nCDA1-VRERBE3
Plasmid#145102PurposeExpressing base editor nCDA1-VRERBE3 in yeast cellsDepositorInsertnCDA1-VRERBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A/D1135V/G1218R/R1335E/T1337R)PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT130_GalL_nCDA1-NGBE3
Plasmid#145094PurposeExpressing base editor nCDA1-NGBE3 in yeast cellsDepositorInsertnCDA1-NGBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT121_GalL_nCDA1-VQRBE3
Plasmid#145086PurposeExpressing base editor nCDA1-VQRBE3 in yeast cellsDepositorInsertnCDA1-VQRBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A, D1135V, R1335Q, T1337R)PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT92_GalL_nCDA1Δ188-BE3
Plasmid#145052PurposeExpresses nCDA1Δ188-BE3 in yeast cellsDepositorInsertnCDA1Δ188-BE3
UseCRISPRTagsExpressionYeastMutationpmCDA1(1-188AA); spCas9(D10A)PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT90_GalL_nCDA1Δ190-BE3
Plasmid#145050PurposeExpresses nCDA1Δ190-BE3 in yeast cellsDepositorInsertnCDA1Δ190-BE3
UseCRISPRTagsExpressionYeastMutationpmCDA1(1-190AA); spCas9(D10A)PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT85_GalL_nCDA1Δ193-BE3
Plasmid#145048PurposeExpresses nCDA1Δ193-BE3 in yeast cellsDepositorInsertnCDA1Δ193-BE3
UseCRISPRTagsExpressionYeastMutationpmCDA1(1-193AA); spCas9(D10A)PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT84_GalL_nCDA1Δ194-BE3
Plasmid#145047PurposeExpresses nCDA1Δ194-BE3 in yeast cellsDepositorInsertnCDA1Δ194-BE3
UseCRISPRTagsExpressionYeastMutationpmCDA1(1-194AA); spCas9(D10A)PromoterGalLAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT78_GalL_nCDA1Δ195-BE3
Plasmid#145044PurposeExpresses nCDA1Δ195-BE3 in yeast cellsDepositorInsertnCDA1Δ195-BE3
UseCRISPRTagsExpressionYeastMutationpmCDA1(1-195AA); spCas9(D10A)PromoterGalLAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIGc-Xph18
Plasmid#133029PurposeExpresses Xph18 in bacteriaDepositorInsertXph18
UseTagsHis10ExpressionBacterialMutationS63KPromoterT7Available sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIGc-Xph20
Plasmid#133024PurposeExpresses Xph20 in bacteriaDepositorInsertXph20
UseTagsHis10ExpressionBacterialMutationS63KPromoterT7Available sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIGc-Xph15
Plasmid#133030PurposeExpresses Xph15 in bacteriaDepositorInsertXph15
UseTagsHis10ExpressionBacterialMutationS63KPromoterT7Available sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-LacI-LAP-HEC1
Plasmid#114033PurposeProduction of baculovirus with LacI-LAP-HEC1DepositorInsertLacI-LAP-HEC1 (NDC80 Human)
UseTagsExpressionInsectMutationnonePromoterCMVAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only