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Plasmid#31326DepositorInsertstathmin 1/oncoprotein 18 (STMN1 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046806
Plasmid#145746PurposeBacterial Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac-His-Flag-Timeless
Plasmid#22892DepositorInsertTimeless (TIMELESS Human)
UseTagsFlag and HisExpressionInsectMutationPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
GFP/WT SP M85
Plasmid#89317PurposeExpression of mouse GFP/WT spastin M85 in mammalian cellsDepositorInsertSPAST M85 (Spast Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJL017
Plasmid#53532PurposeThis plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA geneDepositorInsertetpBA
UseTags6His and mycExpressionBacterialMutationPromoterpBADAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-EPSIN 1
Plasmid#22225DepositorInsertEpsin 1 (Epn1 Rat)
UseTagsXpressExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFSW FLEx mCherry-KASH
Plasmid#139652PurposeConditional expression of mCherry-KASHDepositorInsertmCherry-KASH
UseTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
SENP2M497A
Plasmid#61088Purposecleave the SUMO off when use PETSUMO vector for express fusion proteinDepositorInsertSENP2M497A catalytic domain (SENP2 Human)
UseTagsHisExpressionBacterialMutationM497A; Catalytic domain is residues 364-589PromoterT7Available sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEBG-TFII-I gamma
Plasmid#22156DepositorInsertTFII-I (GTF2I Human)
UseTagsGST and HisExpressionMammalianMutationPromoterAvailable sinceDec. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
c-myc promoter (Frag B)
Plasmid#16606DepositorInsertc-myc promoter (Frag B) (MYC Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationFragB (KpnI->AccI). The KpnI site is at -1194 …PromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
c-myc promoter (Frag C)
Plasmid#16607DepositorInsertc-myc promoter (Frag C) (MYC Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationFragC (AccI->PvuII). The AccI site is at -741 …PromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pMH4
Plasmid#52529Purposeserves as PCR-template to generate HR donors for C-terminal 2xFLAG-tagDepositorInsert2x FLAG-tag
UseBlunt cloning kitTagsExpressionMutationPromoterAvailable sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-ShoHELIX-KanR (BO4)
Plasmid#181795PurposepDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargoDepositorInsertKanR, R6K origin of replication
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
p6621 MSCV-IP N-HAonly SRC
Plasmid#35017DepositorInsertSRC (SRC Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceFeb. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCfB5191
Plasmid#126911PurposePlasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiaeDepositorInsertX-4 and XII-5 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046764
Plasmid#145673PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST-hPDIb′a′
Plasmid#60556Purposeenhance solubility and correct disulfide bond-formation of fused protein expressed in E.coliDepositorTypeEmpty backboneUseTagsHis6-hPDIb'a' tagExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mClover3
Plasmid#171497PurposeT7 promotor drives in vitro transcription of mClover3 mRNADepositorInsertmClover3
UsePgehmeTagsExpressionMutationPromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB700 lenti gRNA EYFP
Plasmid#167906PurposeLentiviral vector for expressing U6 sgRNA cloning plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only