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Plasmid#217827PurposeStuffer plasmid with golden gate compatible bidirectional promoterDepositorInsertGal1/Gal10 Bidirectional Promoter
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
eSrtA-MT4
Plasmid#200306PurposeBacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT4. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtADepositorInserteSrtA-LPETG-MT4 (MT4 Human)
UseTagsHis-tag and SUMO-tagExpressionBacterialMutationPromoterT7Available sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-Hs4EBP1_50-83-GB1_AA
Plasmid#148275PurposeBacterial Expression of Hs4EBP1_50-83DepositorInsertHs4EBP1_50-83 (EIF4EBP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-Hs4EBP1_50-83-L75AV81A-GB1_AA
Plasmid#148276PurposeBacterial Expression of Hs4EBP1_50-83-L75AV81ADepositorInsertHs4EBP1_50-83-L75AV81A (EIF4EBP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-Hs4EBP1_50-83-Y54AL59A-GB1_AA
Plasmid#148277PurposeBacterial Expression of Hs4EBP1_50-83-Y54AL59ADepositorInsertHs4EBP1_50-83-Y54AL59A (EIF4EBP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-Hs4EBP1_50-83-YLLVtoAAAA-GB1_AA
Plasmid#148278PurposeBacterial Expression of Hs4EBP1_50-83-YLLVtoAAAADepositorInsertHs4EBP1_50-83-YLLVtoAAAA (EIF4EBP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-Hs4EBP3_36-67-GB1_AA
Plasmid#148279PurposeBacterial Expression of Hs4EBP3_36-67DepositorInsertHs4EBP3_36-67 (EIF4EBP3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpH-HseIF4E_36-217_AA
Plasmid#148282PurposeBacterial Expression of HseIF4E_36-217DepositorInsertHseIF4E_36-217 (EIF4E Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmCUP_325-376-R329E-GB1_AA
Plasmid#148263PurposeBacterial Expression of DmCup_325-376-R329EDepositorInsertDmCup_325-376-R329E (cup Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmCUP_325-376-R329ER336E-GB1_AA
Plasmid#148264PurposeBacterial Expression of DmCup_325-376-R329ER336EDepositorInsertDmCup_325-376-R329ER336E (cup Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmCUP_325-376-R329ER336ER375E-GB1_AA
Plasmid#148265PurposeBacterial Expression of DmCup_325-376-R329ER336ER375EDepositorInsertDmCup_325-376-R329ER336ER375E (cup Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmCUP_325-376-R336E-GB1_AA
Plasmid#148266PurposeBacterial Expression of DmCup_325-376-R336EDepositorInsertDmCup_325-376-R336E (cup Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpH-CeeIF4E3_1-215opt_AB
Plasmid#148367PurposeBacterial Expression of CeeIF4E3_1-215DepositorInsertCeeIF4E3_1-215
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpH-CeeIF4E3_30-215opt_AB
Plasmid#148369PurposeBacterial Expression of CeeIF4E3_30-215optDepositorInsertCeeIF4E3_30-215opt
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPO812
Plasmid#195306PurposepBAD322-MycaIDCas-ΔCas6-RGR-lacZs, arapBAD promoter expressing type I-D Cascade of McCAST (Tn7575) without cas6 gene and ribozyme flanked lacZ spacer 5.DepositorInsertMcCAST Cascade Δcas6 and ribozyme flanked lacZ spacer 5
UseTagsExpressionBacterialMutationPromoterArabinose inducible arapBADAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
CapChR1_pUAST-attb
Plasmid#188040PurposeExpresses CapChR1 in DrosophilaDepositorInsertCapChR1
UseTagsnoneExpressionInsectMutationS63D, L132C, T159C and N258E mutations in CrChR2PromoterUASAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHL 990
Plasmid#52992PurposepLtetO-1:DsrA, pCon:RhyB, pLlacO-1:rpoS::gfpDepositorInsertsDsrA sRNA
RhyB sRNA (competitor)
rpoS
UseSynthetic BiologyTagsgfpExpressionBacterialMutationincludes −150 to +30 bp relative to start codonPromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 762
Plasmid#52990PurposepLtetO-1:MicC, pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfpDepositorInsertsMicC sRNA
sodB
ompC
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutation−56 to +141 bp and −81 to +36 bpPromoterpLlacO-1 and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1035
Plasmid#52999PurposepLtetO-1:RhyB, pCon:fhlA::mCherry, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
fhlA
sodB
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutationcontains −309 to +96 bp relative to start codon a…PromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1033
Plasmid#52998PurposepLtetO-1:RhyB, pCon:rpoS::mCherry, pLlacO-1:sodB::gfpDepositorInsertsRhyB sRNA
rpoS
sodB
UseSynthetic BiologyTagsgfp and mCherryExpressionBacterialMutationcontains −150 and +30 bp relative to start codon …PromoterpCon, pLlacO-1, and pLtetO-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only