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Plasmid#176275PurposeViral vector for expression of TdTomato in cells expressing Flp AND NOT Cre driven by the human Synapsin promoter.DepositorInserttdTomato
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterhuman Synapsin IAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 JIP3a
Plasmid#53457Purposeinteracts with MAPK signaling moduleDepositorInsertmitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)
UseTagsT7ExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
FUW-ubiquitin-SV40-GFP
Plasmid#65445PurposeExpression of GFP in mammalian cells. Control vector for FUW-ubiquitin-EphB-SV40-GFP vectorsDepositorInsertSV40-EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterUBQ and SV40Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLHCX-Flag-mPKM2(C358S)
Plasmid#42513DepositorInsertPyruvate Kinase M2 Cys358->Ser
UseRetroviralTagsFlagExpressionMutationChanged Cysteine 358 to SerinePromoterCMVAvailable sinceMarch 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSV-CaMKIV
Plasmid#45062PurposeExpression of CaMKIVDepositorInsertCam Kinase IV (Camk4 Mouse)
UseTagsExpressionMammalianMutation*PromoterRSVAvailable sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBSSK p85 alpha (delta iSH2)
Plasmid#13432DepositorInsertp85 alpha (delta iSH2) (PIK3R1 Human)
UseTagsflagExpressionBacterialMutationDominant negative p85 alpha. The p110 alpha bindi…PromoterAvailable sinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSA3
Plasmid#52263PurposeBacterial expression of human 6His-tagged SUMO3 and SUMO-E1DepositorUseTags6His-tagExpressionBacterialMutationC-terminal gly-glyPromoterT7 and rbsAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
Sema3e(L)-Fc-His
Plasmid#72145PurposeExpresses the Sema3E protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema3e (Sema3e Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-H2B-mClover3
Plasmid#171489PurposeT7 promotor drives in vitro transcription of mClover3-tagged human H2B mRNADepositorInsertH2B (H2BC11 Human)
UsePgehmeTagsmClover3ExpressionMutationPromoterT7Available sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ntn1-AP-His
Plasmid#71978PurposeExpresses the entire Netrin 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertNtn1 (Ntn1 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-MfPylRS (human-opti)
Plasmid#164081PurposeTo express a Methanosarcina flavescens derived PylRS in mammalian cells for genetic code expansionDepositorInsertMfPylRS(human-opti)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-Pom121-mScarlet
Plasmid#171491PurposeT7 promotor drives in vitro transcription of mScarlet-tagged mouse Pom121 mRNADepositorInsertPom121 (Pom121 Mouse)
UsePgehmeTagsmScarletExpressionMutationPromoterT7Available sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLIK TT 3xFLAG SMAD4 neo
Plasmid#83273PurposeLentiviral vector for inducible expression of SMAD4 with 3xFLAG N-terminal tagDepositorInsertSMAD4 (SMAD4 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
4LNDDE488Q-pCDNA3.1(+)
Plasmid#172228PurposeExpresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) in mammalian cellsDepositorInsertLambda N peptide - human ADAR2 Deaminase Domain E488Q
UseTags6xHis and FlagExpressionMammalianMutationchanged the Glutamic acid 488 in the Deaminase Do…PromoterCMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
L-RARS-5
Plasmid#166535PurposescFv of a human scaffold targeting Arginyl-tRNA synthetase. Antigen coverage aa 70-660 of 660DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
L-KARS-5
Plasmid#166530PurposescFv of a human scaffold targeting Lysyl-tRNA synthetase. Antigen coverage aa 1-597 of 597, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
O-DARS-1
Plasmid#166538PurposescFv of a human scaffold targeting Aspartyl-tRNA synthetase. Antigen coverage aa 1-501 of 501, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
O-LARS-15
Plasmid#166542PurposescFv of a human scaffold targeting Leucyl-tRNA synthetase. Antigen coverage aa 260-509 of 1176DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
ALIX(1-702)-mNeonGreen
Plasmid#191193PurposeTruncated form of ALIX that lacks C-terminal proline-rich domain; C-terminally tagged with mNeonGreen.DepositorInsertALIX (1-702) (PDCD6IP Synthetic, Human)
UseTags6xHIS and mNeonGreenExpressionMammalianMutationDeletion of ALIX C-terminal PRD.PromoterCMVAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only