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Plasmid#92040PurposeExpresses mEos3 fusion with dnRac1 (T17N).DepositorInsertRAC1 (RAC1 Human)
UseTagsmEos3ExpressionBacterial, Insect, and Mamm…MutationT17N (dominant negative)PromoterCMVAvailable sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-GEM-GECO1
Plasmid#32463DepositorArticleInsertGEM-GECO1
UseLab constructedTags6x His and modified TorA MNNNDLFQASRRRFLAQLG[G to…ExpressionBacterialMutationGCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…PromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1alpha-HA-TFAP4
Plasmid#110158PurposeLentiviral vector for expression of human TFAP4 in mammalian cellsDepositorInsertTFAP4 (transcription factor AP-4) (TFAP4 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMIG-Flag-RIPK1-D324A
Plasmid#102678Purposeretroviral expression plasmid for RIPK1-D324ADepositorInsertRIPK1 (RIPK1 Human)
UseRetroviralTagsFlag and IRES-GFPExpressionMammalianMutationCHANGE Glutamate 324 to AlaninePromoterunknownAvailable sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSP64T acvr1ba*
Plasmid#185409PurposeConstitutively active form of acvr1ba (Nodal receptor). For in vitro transcription (SP6)DepositorInsertacvr1ba (acvr1ba Zebrafish)
UseIn vitro synthesis of mrnaTagsExpressionMammalianMutationchanged Threonine 206 to Aspartic acidPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Sha2Cas9
Plasmid#192140PurposeExpresses Sha2Cas9, and cloning backbone for sgRNADepositorInsertSha2Cas9
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpeCas9
Plasmid#192139PurposeExpresses SpeCas9, and cloning backbone for sgRNADepositorInsertSpeCas9
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sa-SchCas9
Plasmid#192137PurposeExpresses Sa-SchCas9, and cloning backbone for sgRNADepositorInsertSa-SchCas9
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hM3D(Gq)::RAM-d2tTA
Plasmid#189627PurposeExpresses hM3D(Gq) under the hSyn promoter, and d2tTA under the pRAM (synthetic Fos) promoter. Plasmid 1 for recording Fos expression.DepositorInsertshM3D(Gq)
d2tTA
UseAAVTagsHAExpressionMammalianMutationPromoterhSyn and pRAMAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTYF-super-mfPV-0.8k-Venus-WPRE
Plasmid#156399PurposeExpresses Venus under PV gene promoterDepositorInsertsVenus
Gal4p65
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-Barkor
Plasmid#24402DepositorInsertBarkor (ATG14 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
mEos3-caRac1
Plasmid#92041PurposeExpresses mEos3 fusion with caRac1 (Q61L)DepositorInsertRAC1 (RAC1 Human)
UseTagsmEos3ExpressionBacterial, Insect, and Mamm…MutationQ61L (constitutively active)PromoterCMVAvailable sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Fah_W
Plasmid#163087Purposeexpressing mouse wild type FAH in mammalian cellsDepositorInsertFah_W
UseTagsMyc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFX-β-tubulin-Sirius
Plasmid#175825PurposeExpress Sirius with microtubule-targeting sequence in mammalian cellsDepositorInsertSirius with microtuble-targeting sequence
UseTagshuman tubulin beta 4B class IVb;TUBB4BExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET27b-ecDHFR-N23PP/L28F
Plasmid#118584PurposeBacterial expression of E.coli DHFR N23PP and L28F mutantDepositorInsertDHFR
UseTagsExpressionBacterialMutationL28 mutated to F28, N23 mutated to P23, a proline…PromoterT7Available sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
J23101
Plasmid#55176PurposeVery weak constitutive promoterDepositorInsertJ23101 promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterJ23101Available sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSB115 - pL2_pSB90_fLUC-I_tGFP-I
Plasmid#123190Purposebinary plant vector for transient tGFP (with intron) and fLUC (with intron) expressionDepositorInsert2x35S::tGFP-I::tNOS 2x35S::fLUC-I::tNOS
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFX-β-tubulin-SECFP
Plasmid#175826PurposeExpress SECFP with microtubule-targeting sequence in mammalian cellsDepositorInsertSECFP with microtuble-targeting sequence
UseTagshuman tubulin beta 4B class IVb;TUBB4BExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha1 Pnos:PhiC31int:Tnos (GB1531)
Plasmid#160616PurposeTU for the constitutive expression of Streptomyces phage PhiC31 integrase.DepositorInsertPhiC31
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Flag Smad3 S204A
Plasmid#27114DepositorInsertSmad3 (SMAD3 Human)
UseXenopus/mammalian/avian/zebrafishTagsFlagExpressionMutationS204APromoterAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only