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Plasmid#193839PurposeExpress TadCBEd-V106W (with SpCas9) in mammalian cellsDepositorInsertTadA-CDd V106W-SpCas9 D10A-2xUGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
spNW25
Plasmid#173484PurposeExpresses spNW25 in Bl21DepositorInsertspNW25
UseTagsHistagExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
p4305
Plasmid#198150Purposebacterial expression of sifA::HaloTag fusionDepositorInsertPsifA sifA::L16::HaloTag::HA (sifA Salmonella enterica serovar Typhimurium)
UseTagsHaloTag-HAExpressionBacterialMutationPromoterAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-Halotag-FRB-EX-nes_pLX304
Plasmid#120913PurposeLentiviral vector expressing HA-Halotag-FRB-EX-NES in mammalian cytosolDepositorInsertHA-Halotag-FRB-EX-NES
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
THBS1-bio-His
Plasmid#53417PurposeExpresses full-length Thrombospondin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTHBS1 (THBS1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hERG1a
Plasmid#53051PurposeExpresses alpha subunit of hERG1a K channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
UseXenopus oocyte expressionTagsExpressionMutationPromoterSP6Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
ITGB1
Plasmid#51920PurposeExpresses full-length Integrin beta-1 precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertITGB1 (ITGB1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationStop Codon at amino acid 728, before CD4, bio and…PromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE6
Plasmid#90340PurposeSTAT5 - Stat5 gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterSTAT5Available sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pTriEx-mCherry-PA-Rac1
Plasmid#22027PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Human, Avena sativa (oat))
UseTags6xHis and mCherryExpressionBacterial, Insect, and Mamm…MutationLOV(Leu404-Leu546), Rac1 starts at Ile4 and conta…PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
BC1177-pHR-SFFV-GFP(1-10)-NLS
Plasmid#164039PurposeExpression of GFP1-10 in mammalian cellsDepositorInsertGFP(1-10)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Capping Protein
Plasmid#89950PurposeF-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends.DepositorInsertsUseTags6*HisExpressionBacterialMutationV222IPromoterAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
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miniCMV-tdTomato-tDeg
Plasmid#217521PurposeExpresses tdTomato-tDeg fluorogenic protein in mammalian cells with miniCMV promoterDepositorInserttdTomato-tDeg fluorogenic protein
UseTagsExpressionMammalianMutationPromoterminiCMVAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_LASV_GPC
Plasmid#207287PurposeMammalian expression of Lassa virus GPC proteinDepositorInsertLASV_GPC
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSP1E2D1
Plasmid#161606PurposeBacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formationDepositorInsertMSP1E2D1
UseTags7-HisExpressionBacterialMutation"extended" MSP1D1; contains repeat of h…PromoterT7Available sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-dV-IgG1/λ
Plasmid#52214PurposeHuman immunoglobulin gamma 1 antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) coloniesDepositorInsertsHuman immunoglobulin gamma 1 constant region
Human immunoglobulin lambda constant region
UseTagsExpressionMammalianMutationDeleted Variable regionPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+30)
Plasmid#199164Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+30)
UseTagsMGHHHHHHGGAExpressionMutationE16R, V21K, D29K, E42K, E44K, D86K, E100K, D112K,…PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pyEvolvR (enCas9-PolI5M)
Plasmid#158073PurposeExpresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.Cre.WPRE.hGH
Plasmid#105550PurposeAAV expression of Cre from GFAP promoterDepositorHas ServiceAAV PHP.eB and AAV5InsertCre
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterGFAPAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only