Addgene

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Showing: 8021 - 8040 of 36897 results

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pcDNA TMEM 138-flag

Plasmid

#41628

Depositor

Joseph Gleeson

Article

Lee et al Science. 2012 Feb 24;335(6071):966-9. Ep

Insert

Transmembrane Protein 138 (TMEM138 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2013

Availability

Academic Institutions and Nonprofits only
p-miR-Report + MT miR-181 BS in CDX2 3UTR

Plasmid

#22793

Depositor

Xin Wang

Article

Ji et al Hepatology. 2009 Aug . 50(2):472-80.

Insert

Mutant miR-181 Binding Site in Caudal type homeobox transcription factor 2 3 Untranslated Region (CDX2 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 30, 2010

Availability

Academic Institutions and Nonprofits only
pSB61 - pL0_V2 (CDS1)

Plasmid

#123183

Purpose

Golden Gate (MoClo; CDS1) compatible Tomato yellow leaf curl virus (TYLCV) V2 silencing suppressor based on NCBI Reference Sequence NC_004005

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

Tomato yellow leaf curl virus (TYLCV) V2 (v2 Synthetic)

Use

Part for plant expression

Tags

Expression

Plant

Mutation

No BpiI and BsaI sites

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_hE2F7mg_6ntmut

Plasmid

#73075

Purpose

human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

E2F7 (E2F7 Human)

Use

Tags

Expression

Mammalian

Mutation

partial gene, exons 11,12,13 spaced by introns wi…

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_hE2F7mg_10ntmut

Plasmid

#73076

Purpose

human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

E2F7 (E2F7 Human)

Use

Tags

Expression

Mammalian

Mutation

partial gene, exons 11,12,13 spaced by introns wi…

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL c2x Tral FL-15D

Plasmid

#113010

Purpose

For bacterial expression of MBP fusion of full-length Drosophila Tral with Phos-mimic mutations

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length tral (tral Fly)

Use

Tags

Expression

Bacterial

Mutation

15 PNG phosphorylation sites mutated to D

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUDP046

Plasmid

#107062

Purpose

Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting KU80 from Ogataea parapolymorpha

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

HH-gRNA-HDV targetting OpKU80 inO. parapolymorpha

Use

Tags

Expression

Yeast

Mutation

Promoter

ScTDH3

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-vsv-Incenp d543-746-GFP

Plasmid

#108503

Purpose

inducible expression of vsv-INCENP d543-746-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

INCENP d543-746 (INCENP Human)

Use

Tags

EGFP and VSV

Expression

Mammalian

Mutation

delta alpha helix

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-vsv-Incenp 8KR-GFP

Plasmid

#108505

Purpose

inducible expression of vsv-INCENP 8KR-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

INCENP (INCENP Human)

Use

Tags

EGFP and VSV

Expression

Mammalian

Mutation

K548R, K563R, K602R, K609R, K614R, K622R, K630R, …

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-vsv-Incenp 8KQ-GFP

Plasmid

#108506

Purpose

inducible expression of vsv-INCENP 8KQ-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

INCENP (INCENP Human)

Use

Tags

EGFP and VSV

Expression

Mammalian

Mutation

K548Q, K563Q, K602Q, K609Q, K614Q, K622Q, K630Q, …

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pAc-GFP-dSUMOdeltaC

Plasmid

#85697

Purpose

expression of GFP-SUMO1 delta C in Drosophila cells

Depositor

Licio Collavin, Giannino Del Sal

Article

Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

Insert

SUMO (Sumo Fly)

Use

Tags

GFP

Expression

Insect

Mutation

non-conjugatable SUMO, lacking the C-terminal gly…

Promoter

pAc5

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
NPR2 gRNA (BRDN0001148355)

Plasmid

#77753

Purpose

3rd generation lentiviral gRNA plasmid targeting human NPR2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NPR2 (NPR2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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AK7 gRNA (BRDN0001145278)

Plasmid

#77913

Purpose

3rd generation lentiviral gRNA plasmid targeting human AK7

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AK7 (AK7 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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TNNI3K gRNA (BRDN0001147407)

Plasmid

#77898

Purpose

3rd generation lentiviral gRNA plasmid targeting human TNNI3K

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FPGT-TNNI3K,TNNI3K (FPGT-TNNI3K, TNNI3K Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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IP6K2 gRNA (BRDN0001145651)

Plasmid

#77819

Purpose

3rd generation lentiviral gRNA plasmid targeting human IP6K2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

IP6K2 (IP6K2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BRSK1 gRNA (BRDN0001145478)

Plasmid

#77786

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRSK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRSK1 (BRSK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAST4 gRNA (BRDN0001146863)

Plasmid

#76883

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAST4

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAST4 (MAST4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDK11A gRNA (BRDN0001147530)

Plasmid

#75892

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDK11A

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDK11A (CDK11A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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STKLD1 gRNA (BRDN0001162456)

Plasmid

#75813

Purpose

3rd generation lentiviral gRNA plasmid targeting human STKLD1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STKLD1 (C9orf96 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDC42BPA gRNA (BRDN0001147767)

Plasmid

#75748

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDC42BPA

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDC42BPA (CDC42BPA Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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