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Plasmid#87889Purposeresearch of alternative isoforms of the protein dysferlinDepositorInsertDYSF, alt exon 1, 5a, 40a (DYSF Human)
UseTagsExpressionBacterialMutationalternative isoforms, see commentsPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-P70a-Top-T~P70a-Bottom-T
Plasmid#87314PurposeExpresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.DepositorInsertSplit-Broccoli system
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Rluc-BoxB-A114-N40
Plasmid#50562PurposeTemplate DNA for in vitro transcription using T7 polymeraseDepositorInsertLuciferase-BoxB-A114-N40
UseCloning vectorTagsExpressionMutationPromoterlacAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Split-Broccoli-Toehold-Switch
Plasmid#87316PurposeExpresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry.DepositorInsertSplit-Broccoli system and RNA Trigger (TS2_AT01) Toehold (TS2_KS01) switch for mCherry
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIG-746pUC19-tNGFR-P2A-CD5
Plasmid#186121PurposeKnockin of truncated NGFR to target geneDepositorInsertCD5 (CD5 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19, containing H2-Kb c57/BL6 WK9
Plasmid#102641PurposeEncodes sequence of the murine H2-Kb alleleDepositorInsertH2-Kd Balb/c
UseTagsExpressionMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIG-738pUC19-tNGFR-P2A-Kindlin-3
Plasmid#186092PurposeKnockin of truncated NGFR to target geneDepositorInsertKINDLIN3 (FERMT3 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-707pUC19-tNGFR-P2A-TTC37
Plasmid#186122PurposeKnockin of truncated NGFR to target geneDepositorInsertTTC37 (SKIC3 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-736pUC19-tNGFR-P2A-CD40L
Plasmid#186104PurposeKnockin of truncated NGFR to target geneDepositorInsertCD40L (CD40LG Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)
Plasmid#133793PurposeU6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017DepositorInsertCjeCas9 sgRNA entry vector
UseTagsExpressionMammalianMutationV2-TGAA linker sgRNA architecture from Kim et al.…PromoterU6Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCIDT-attL1-Worm ABeta-attR5
Plasmid#160437PurposeEntry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombinationDepositorInsertHomo sapiens amyloid beta precursor protein (APP Nematode, Human)
UseTagsCleavage signalExpressionBacterialMutationCodon-optimized for expression in C. elegansPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUCHR-CD5Flag2-P2A-smAID-mClover
Plasmid#110657PurposeLentiviral vector expressing cell surface Flag-tag and monomeric GFP N-terminaly fused with auxin inducible degronDepositorInsertCD5Flag2-P2A-AID-mClover
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57 DYSF, alt exon 1a, 5a, 40a
Plasmid#87897Purposeresearch of alternative isoforms of the protein dysferlinDepositorInsertDYSF, alt exon 1a, 5a, 40a (DYSF Human)
UseTagsExpressionBacterialMutationalternative isoforms, see commentsPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Rluc-let-7-A114-N40
Plasmid#50563PurposeTemplate DNA for in vitro transcription using T7 polymeraseDepositorInsertLuciferase-let7-A114-N40
UseCloning vectorTagsExpressionMutationPromoterlacAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC-DEST-hSOX2-EGFP-PuroTK
Plasmid#141026PurposeReporter integrationDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-dual-scafffold-2.0 (pBP49)
Plasmid#218931PurposeThe dual-scaffold for making dual-CRISPR screening libraries or dual-CRISPR plasmids.DepositorInsertdual scaffolds for CRISPR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-dCas9
Plasmid#218371PurposeExpression of dCas9 for CRISPR-interferenceDepositorInsertdCas9
UseCRISPRTagsExpressionBacterialMutationPromoterPtetAAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-lacZ
Plasmid#218370PurposeEmpty backbone for lacZ fusionsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-tRNAGln-BbsI-sU6-Kan
Plasmid#213016PurposeVector for Cloning of multiple gRNAs driven by distinct promoters (tRNA-Gln and synthetic U6)DepositorInserttRNAGln promoter, sU6 promoter, gRNA scaffolds
UseTagsExpressionMutationPromotertRNAGln promoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56
Plasmid#208645PurposeRemove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.DepositorInsertTwin Strep tag with RPL3 genome HomoArm (RPL3 Synthetic, Human)
UseCRISPR; Ssdna donnor preparation for knock-in of…TagsTwin StrepExpressionMutationTwo SNP (rs749555789, rs760194863) were introduce…PromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only