-
Plasmid#149613PurposegRNA expression in B. burgdorferi, parental vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only
-
pBAD-HisD-miniGFP1
Plasmid#188896PurposeExpress 6xHis-Tag miniGFP1 in E.coliDepositorInsertminiGFP1
UseTags6xHisExpressionBacterialMutationPromoteraraBADAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sp_PE
Plasmid#212038PurposeBacterial expression of the PE2 proteins for genome editing in E. coliDepositorInsertPE2
UseCRISPRTagsExpressionMutationPromoterL-arabinose-inducible promoter PBADAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-entry-puro
Plasmid#85745PurposeLentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS159
Plasmid#122378PurposeCRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
TOPO mKate2
Plasmid#68441PurposeTOPO construct expressing mKate2 for generation of GMAP-compatible geneDepositorInsertmKate2
UseGmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
Frb-N-TEV
Plasmid#100095PurposeSNIPer plasmid, N terminal TEV fused to FrbDepositorInsertN-TEV
UseRetroviralTagsFrb and MycExpressionMammalianMutationN terminal domainPromoterCMVAvailable sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-CreiLOV
Plasmid#192817PurposeExpresses CreiLOV in bacteriaDepositorInsertCreiLOV
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FKBP-C-TEV 219
Plasmid#100096PurposeSNIPer plasmid, C terminal TEV fused to FKBPDepositorInsertC-TEV
UseRetroviralTagsFKBP and MycExpressionMammalianMutationC terminal domainPromoterCMVAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMEH381 VSV-G MS2 HA
Plasmid#168223PurposeMammalian expression of vesicular stomatitis virus glycoprotein fused to the MS2 dimer and C-terminal HA tagDepositorInsertVSV-G
UseTagsHA and MS2 dimerExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAT75
Plasmid#204306PurposeFlippase under heat shock control. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthpartsDepositorInsertie1-EGFP
UseCRISPRTagsExpressionMutationPromoterie1Available sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT39
Plasmid#204289PurposeFor marking CRISPR HDR integrant with EGFP expressed in flight muscles.DepositorInsertMHC-EGFP+H3:H26
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-13
Plasmid#119992PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mCherryDepositorInsertmCherry
UsePcr templateTagsmCherryExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS143
Plasmid#122377PurposeCRISPRi plasmid for use in Candida albicans. Contains dCas9 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)DepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS263
Plasmid#122379PurposeCRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codonDepositorInsertHSP90
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS267
Plasmid#122380PurposeCRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codonDepositorInsertHSP90
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBR_attB(bxb)_ccdB_lox_v2
Plasmid#198040PurposeBxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)DepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBDEST.B
Plasmid#79460PurposePromoterless, Sleeping Beauty-compatible Gateway destination vector, Blasticidin selectionDepositorTypeEmpty backboneUsePromoterless gateway destination vectorTagsExpressionMutationPromoternoneAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN_TmiRc3
Plasmid#25748PurposeEntry vector for cloning miR30-based shRNA driven by TRE promoter.DepositorTypeEmpty backboneUseEntry vectorTagsExpressionMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
IVT-MCP-Phi29
Plasmid#207456Purposein vitro transcription of mRNADepositorInsertMCP-Phi29
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only