We narrowed to 3,848 results for: golden gate

1. pLPP9

   Plasmid

   #209971

   Purpose

   Contains Level 0 Part: constitutive Promoter (PldhD) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   Promoter (P_ldhD)
   

   Expression

   Bacterial

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pLPS2

   Plasmid

   #209972

   Purpose

   Contains Level 0 Part: ribosome binding site (L. plantarum optimized) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   RBS (Lp)
   

   Expression

   Bacterial

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pLPT2

   Plasmid

   #209974

   Purpose

   Contains Level 0 Part: Terminator (TpepN) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   Terminator (T_pepN)
   

   Expression

   Bacterial

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLPR1

   Plasmid

   #209979

   Purpose

   Contains Level 0 Part: resistance cassette (ermBL) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   resistance cassette (ermBL)
   

   Expression

   Bacterial

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLP3

   Plasmid

   #209958

   Purpose

   Entry vector to store Level 0 broad-host range L. plantarum replication origin parts

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pLPP1

   Plasmid

   #209963

   Purpose

   Contains Level 0 Part: mock / negative control Promoter (P-nc) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   Promoter (mock)
   

   Expression

   Bacterial

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pLPP2

   Plasmid

   #209964

   Purpose

   Contains Level 0 Part: constitutive Promoter (P48) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   Promoter (P48)
   

   Expression

   Bacterial

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pLPC31

   Plasmid

   #209996

   Purpose

   Contains Level 0 Part: 3' Connector (3C2SN) for the construction of Level 1 plasmids

   Depositor

   Georg Fritz, Thorsten Mascher

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   3' Connector (3C2SN)
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLPC32

   Plasmid

   #209997

   Purpose

   Contains Level 0 Part: 3' Connector (3C5CSN) for the construction of Level 1 plasmids

   Depositor

   Georg Fritz, Thorsten Mascher

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   3' Connector (3C5CSN)
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLPC52

    Plasmid

    #209991

    Purpose

    Contains Level 0 Part: 5' Connector (5C2SN) for the construction of Level 1 plasmids

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    5' Connector (5C2SN)
    

    Expression

    Bacterial

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLPC51

    Plasmid

    #209990

    Purpose

    Contains Level 0 Part: 5' Connector (5C1CSN) for the construction of Level 1 plasmids

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Insert

    5' Connector (5C1CSN)
    

    Expression

    Bacterial

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDM098

    Plasmid

    #216818

    Purpose

    Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for C-terminal tagging.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Insert

    mStayGold
    

    Tags

    FLAG-(SGGS)x2-XTEN16-(GGGGS)x3

    Expression

    Bacterial

    Mutation

    Aspergillus nidulans codon-adjusted

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMA-SpCas9-g10

    Plasmid

    #80793

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMA-SpCas9-g9

    Plasmid

    #80792

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMA-SpCas9-g8

    Plasmid

    #80791

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEM-T Easy-AtpU6-26

    Plasmid

    #160218

    Purpose

    AtpU6-26 Golden Gate level 0 piece to express gRNAs

    Depositor

    Matias Kirst

    Article

    Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

    Insert

    pAtU6-26 promoter
    

    Use

    Golden gate level 0 piece to express grnas

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. BB2_EF

    Plasmid

    #98538

    Purpose

    empty BB2 for transcription unit cloning

    Depositor

    Brigitte Gasser, Diethard Mattanovich, Michael Sauer

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBT100-neo

    Plasmid

    #123137

    Purpose

    Golden Gate donor vector for the assembly of EGFP-based mammalian recombinase reporters.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Neomycin, poly-A terminator
    

    Use

    Synthetic Biology; Golden gate donor vector

    Promoter

    none

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HSA_3b*_AP

    Plasmid

    #238899

    Purpose

    Plasmid insert contains a gene sequence for Human serum albumin for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    Recombinant Human Serum Albumin (HSA) 3b*
    

    Use

    Synthetic Biology

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. C-term_TEV_aMFx1 (4a)

    Plasmid

    #238903

    Purpose

    Plasmid insert contains the aMFx1 C-terminal tag sequence with the TEV cleavage site for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    C-term_TEV_aMFx1
    

    Use

    Synthetic Biology

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge