We narrowed to 3,761 results for: golden gate

1. pLPP1

   Plasmid

   #209963

   Purpose

   Contains Level 0 Part: mock / negative control Promoter (P-nc) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   Promoter (mock)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pLPP2

   Plasmid

   #209964

   Purpose

   Contains Level 0 Part: constitutive Promoter (P48) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   Promoter (P48)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pLPC31

   Plasmid

   #209996

   Purpose

   Contains Level 0 Part: 3' Connector (3C2SN) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher, Georg Fritz

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   3' Connector (3C2SN)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLPC32

   Plasmid

   #209997

   Purpose

   Contains Level 0 Part: 3' Connector (3C5CSN) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher, Georg Fritz

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   3' Connector (3C5CSN)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLPC52

   Plasmid

   #209991

   Purpose

   Contains Level 0 Part: 5' Connector (5C2SN) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher, Georg Fritz

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   5' Connector (5C2SN)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLPC51

   Plasmid

   #209990

   Purpose

   Contains Level 0 Part: 5' Connector (5C1CSN) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher, Georg Fritz

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   5' Connector (5C1CSN)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDM098

   Plasmid

   #216818

   Purpose

   Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for C-terminal tagging.

   Depositor

   Ken Sawin

   Article

   Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

   Insert

   mStayGold
   

   Use

   Tags

   FLAG-(SGGS)x2-XTEN16-(GGGGS)x3

   Expression

   Bacterial

   Mutation

   Aspergillus nidulans codon-adjusted

   Promoter

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMA-SpCas9-g10

   Plasmid

   #80793

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMA-SpCas9-g9

   Plasmid

   #80792

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMA-SpCas9-g8

    Plasmid

    #80791

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEM-T Easy-AtpU6-26

    Plasmid

    #160218

    Purpose

    AtpU6-26 Golden Gate level 0 piece to express gRNAs

    Depositor

    Matias Kirst

    Article

    Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

    Insert

    pAtU6-26 promoter
    

    Use

    Golden gate level 0 piece to express grnas

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. BB2_EF

    Plasmid

    #98538

    Purpose

    empty BB2 for transcription unit cloning

    Depositor

    Michael Sauer, Brigitte Gasser, Diethard Mattanovich

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBT100-neo

    Plasmid

    #123137

    Purpose

    Golden Gate donor vector for the assembly of EGFP-based mammalian recombinase reporters.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Neomycin, poly-A terminator
    

    Use

    Synthetic Biology; Golden gate donor vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HSA_3b*_AP

    Plasmid

    #238899

    Purpose

    Plasmid insert contains a gene sequence for Human serum albumin for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    Recombinant Human Serum Albumin (HSA) 3b*
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. C-term_TEV_aMFx1 (4a)

    Plasmid

    #238903

    Purpose

    Plasmid insert contains the aMFx1 C-terminal tag sequence with the TEV cleavage site for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    C-term_TEV_aMFx1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. C-term_C-myc (4a)

    Plasmid

    #238907

    Purpose

    Plasmid insert contains the C-myc C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    C-term_C-myc
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SP_H3

    Plasmid

    #228189

    Purpose

    Plasmid contains the secretion signal peptide sequence of the yeast CIS3 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    sp_P47001_CIS3_YEAST:0-21
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SP_H4

    Plasmid

    #228190

    Purpose

    Plasmid contains the secretion signal peptide sequence of the human CCL8 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    sp_P80075_CCL8_HUMAN:0-23
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SP_H5

    Plasmid

    #228191

    Purpose

    Plasmid contains the secretion signal peptide sequence of the human SDC4 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    sp_P31431_SDC4_HUMAN:0-20
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SP_H6

    Plasmid

    #228192

    Purpose

    Plasmid contains the secretion signal peptide sequence of the human BPIB4 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

    Depositor

    Joseph Brock

    Article

    Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

    Insert

    sp_P59827_BPIB4_HUMAN:0-17
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge