-
Plasmid#32083DepositorInsertMenin isoform 2 (MIR302B Human)
UseTagsFlagExpressionMammalianMutationS543APromoterCMVAvailable sinceFeb. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcFLAG-Grap2-WT
Plasmid#74511PurposeOverexpression of Grap2 in mammalian cellsDepositorInsertGrap2 (GRAP2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pK-SAN1-Flag
Plasmid#117163PurposeExpresses Flag-tagged SAN1 nuclease in mammalian cells.DepositorInsertSAN1 (FAM120B Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-S650D
Plasmid#171246PurposeMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Asp (AR-S650D)DepositorInsert3xFlag-tagged human androgen receptor Ser650Asp mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Ser650Asp (AR-S650D)PromoterCMVAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-M4-AR-S650A
Plasmid#171245PurposeMammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Ala (AR-S650A)DepositorInsert3xFlag-tagged human androgen receptor Ser650Ala mutant (AR Human)
UseTagsFlagExpressionMammalianMutationAR-Ser650Ala (AR-S650A)PromoterCMVAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
GAL FLAG46QDProCFPp303
Plasmid#15585DepositorInserthtt 46Q Delta Pro (HTT Human)
UseTagsCFP and FLAGExpressionYeastMutationProline-rich region removedPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLKO_V5.FLAG.VSVg_NGFR
Plasmid#158247PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsV5.FLAG.VSVgExpressionMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO_NWS.V5.FLAG_NGFR
Plasmid#158251PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsNWS.V5.FLAGExpressionMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS_FLAG-G1 PylRS
Plasmid#154763Purposeplasmid with G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integrationDepositorInsertG1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
UseTagsFLAGExpressionMammalianMutationPromoterEF1Available sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-S6-FLAG:kanMX6
Plasmid#134438PurposePCR-based C-terminal S6-FLAG tagDepositorInsertS6-FLAG
UsePcr-based tagging in yeastTagsExpressionMutationPromoterAvailable sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFRT-TODestFLAGHA_RBPMS_KDR_HindIII
Plasmid#65662PurposeFLAG-HA expression vector with RBPMS carrying mutation to introduce HindIII site and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationintroduced HindIII site (final goal to exchange F…PromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-FLAGNDHFR
Plasmid#63979PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsFLAG; TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-S-P1-FLAG
Plasmid#177095PurposeP1 variant S-protein with c-terminal FLAG tagDepositorInsertS-protein P1 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDESTFLAGHApuro3-Ago2K533AK570A
Plasmid#14104DepositorInsertAgo2 (AGO2 Human)
UseTagsFLAG/HAExpressionMammalianMutationchanged Lys533 to Ala, Lys570 to AlaninePromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc1-FLAGDHFR
Plasmid#63938PurposePosition 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsFLAGExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFlagCMV2-UbcM8C86A
Plasmid#12441DepositorInsertUbcM8 (Ube2l6 Mouse)
UseTagsFLAGExpressionMammalianMutationC86APromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-3xflagGREB1c
Plasmid#112223Purposemammalian expression of flag tagged GREB1cDepositorInsertGREB1c
UseTags3x flagExpressionMammalianMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2Flag CMV2-ZO-2-N
Plasmid#27418DepositorInsertZonula Occludens 2 (TJP2 Human)
UseTags2xFlagExpressionMammalianMutationDeletion of the carboxy-terminus of ZO-2, so only…PromoterAvailable sinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
EZH2-TALEN_Right_No_FLAG
Plasmid#79900PurposeUntagged EZH2-TALEN_Right to target the N-terminus of human EZH2DepositorInsertEZH2-TALEN_Right_No_FLAG (EZH2 Human)
UseTALENTagsExpressionMutationPromoterAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTLPflaG-small
Plasmid#45476DepositorInsertflaA-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 4, 2013AvailabilityAcademic Institutions and Nonprofits only