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Plasmid#156416Purposekdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A-G12V with an alpha-crystallin mCerulean reporter flanked by Tol2 sitesDepositorInserthuman mutant KRAS4A-G12V (KRAS Human)
UseZebrafish transgenesisTagsmScarlet-IExpressionMutationmutant KRAS4A-G12VPromoterkdrlAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
H2B-mScarlet- hMeikin(1-332, E151R, R154E)-mNeonGreen
Plasmid#174715PurposeStable expression of fluorescent Separase cleavage sensor with human Meikin with Separase-resistant mutationsDepositorInsertMeikin
UseRetroviralTagsH2B-mScarlet and mNeonGreenExpressionMammalianMutation1-332, E151R, R154EPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
JDW 858 (pTol2-Kdrl-V5-mScarlet-KRAS4A-WT--AC-Cerulean)
Plasmid#180468Purposekdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A with an alpha-crystallin mCerulean reporter flanked by Tol2 sitesDepositorInsertKRAS4A (KRAS Human)
UseZebrafish transgenesisTagsV5-mScarlet-IExpressionMutationPromoterkdrlAvailable sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
hTMEM115-TEV-3xHA-P2A-mScarlet HDR template
Plasmid#214998PurposeEndogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.DepositorInserthTMEM115-TEV-3xHA-P2A-mScarlet HDR template (TMEM115 Human)
UseHdr templateTags3xHAExpressionMutationPromoterNoneAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p254-RV-CAG-dio-mScarlet-T2A-Dach1-202
Plasmid#204742PurposeConditional expression of target gene Dach1 with fluorescent reporter mScarletDepositorInsertDach1
UseRetroviralTagsExpressionMutationPromoterCAGAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
iF60 PB-LoxStopLox-Zim3-dCas9-mScarlet-puro-BFP
Plasmid#204729PurposeCre-inducible CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
iF57 PB-FrtStopFrt-Zim3-dCas9-mScarlet-puro-BFP
Plasmid#204727PurposeFrt-inducible CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
iE61 PB-Zim3-XTEN-dCas9-mScarlet-puro-BFP
Plasmid#204722PurposeConstitutive CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
iF51 PB-Zim3-dCas9-mScarlet-Hygro-Neo-SnapTag
Plasmid#204725PurposeConstitutive CRISPRi by Zim3-dCas9InsertZim3-dCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAH45 - MosTI [unc-119(partial) | MCS | wrmScarlet]
Plasmid#200365PurposeCloning vector for mosTI(unc-119, wrmScarlet). MCS or Golden Gate.DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mScarlet-I-mNeonGreen-ADH1term-TEFpr-hphΔC (pKBJ011)
Plasmid#173462PurposeC-SWAT donor for Selection Reconstitution tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-I-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEarleyGate100-pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
Plasmid#140170PurposeExpresses a regulatable secretory cargo for Arabidopsis thalianaDepositorInsertpSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PARL-FLAG-CT S277G
Plasmid#13615DepositorUseTagsFLAGExpressionMammalianMutationChanged catalytic Serine 277 to AlaninePromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PARL-FLAG-CT L79E
Plasmid#13640DepositorUseTagsFLAGExpressionMammalianMutationchanged Leu 79 to GluPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PARL-FLAG-CT H335G
Plasmid#13749DepositorUseTagsFLAGExpressionMammalianMutationChanged catalytic His 335 to GlyPromoterAvailable sinceFeb. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
ARL5:Q15-L176 (2H17)
Plasmid#25527PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertARL5:Q15-L176 (ARL5A Human)
UseTagsHisExpressionBacterialMutationContains amino acids Q15-L176PromoterAvailable sinceAug. 24, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3 PARL-FLAG-CT S70A
Plasmid#13620DepositorUseTagsFLAGExpressionMammalianMutationchanged Serine 70 to AlaPromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
ARL5:H14-K177 (2H16)
Plasmid#25526PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertARL5:H14-K177 (ARL5A Human)
UseTagsHisExpressionBacterialMutationContains amino acids H14-K177PromoterAvailable sinceAug. 24, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3 PARL-FLAG-CT S65A
Plasmid#13618DepositorUseTagsFLAGExpressionMammalianMutationchanged Serine 65 to AlaninePromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PARL-FLAG-CT T69A
Plasmid#13619DepositorUseTagsFLAGExpressionMammalianMutationchanged Thr 69 to AlaPromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only