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Plasmid#157603PurposeMammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertPAPLN (PAPLN Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceFeb. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-tdTomato targeting vector (compatible with CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA)
Plasmid#194728PurposeAAVS1-tdTomato targeting vector, compartible with Cpf1DepositorArticleInserttdTomato
UseCRISPR; Knockin targeting plasmidTagsExpressionMutationPromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBig2ab zz TEV YBBR POT1 ZZ TEV TPP1 MBP TEV TIN2 ZZ TEV TRF1 (4comp1)
Plasmid#185447PurposeCoexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF1 and TPP1 each with a zz tag and TEV site, and human TIN2 with an MBP affinity tag and TEV site in insect cellsDepositorUseTagsMBP, YBBR, and ZZExpressionInsectMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBig1a zz TEV YBBR POT1 MBP TEV TPP1 MBP TEV TIN2 ZZ TEV TRF2 (4comp2)
Plasmid#185448PurposeCoexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF2 with a zz tag and TEV site, and human TIN2 and TPP1 each with an MBP affinity tag and TEV site in insect cellsDepositorUseTagsMBP, YBBR, and ZZExpressionInsectMutationSee Depositor Comments BelowPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Compact mismatched sgRNA library for gene titration (human essential genes, CRISPRi)
Pooled Library#136479PurposeExpresses gRNAs for gene titration of ~2400 human genes using CRISPRiDepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
UbiGate Components (set1)
Plasmid Kit#1000000144PurposeGenerate bacterial expression cassettes of up to six ORFs simultaneously; also contains ubiquitin, the E1 UBA1, an E2 collection, and a selection of E3s and their substrates for ubiquitination assaysDepositorAvailable sinceFeb. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2 Gateway compatible toolbox for the study of the nervous system and neurodegenerative disease
Plasmid Kit#1000000087PurposeTol2 Zebrafish toolkit: 4 promoters, 6 fluorophores, MultiSite Gateway (Invitrogen) compatible and cloning sites. Production transgenic zebrafish for neurodegenerative disordersDepositorAvailable sinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
High Complexity Golden Gate Assembly Standards Kit (24 fragment lac cassette)
Plasmid Kit#1000000221PurposeA positive control for high-complexity golden gate assembly. Junction sites were predicted using comprehensive end-joining ligation fidelity and bias data to allow high accuracy assembly.DepositorAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
zCas9i cloning kit: MoClo-compatible CRISPR/Cas9 cloning kit with intronized Cas9
Plasmid Kit#1000000171PurposeVectors and DNA modules for assembly of CRISPR/Cas9 constructs for use in plants. The constructs are made with zCas9i, a high efficiency intron-optimized SpCas9-coding gene.DepositorAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
C321.∆A T7RNAP ompT- rne-
Bacterial Strain#182777PurposeRecoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT and rne inactivatedDepositorBacterial ResistanceSpectinomycinAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
C321.∆A ompT- rne- endA-
Bacterial Strain#182774PurposeRecoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA inactivatedDepositorBacterial ResistanceAmpicillinAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
C321.∆A ompT- rne- lon-
Bacterial Strain#182773PurposeRecoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, lon inactivatedDepositorBacterial ResistanceAmpicillinAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
C321.∆A T7RNAP ompT- rne- lon-
Bacterial Strain#182778PurposeRecoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT, rne, and lon inactivatedDepositorBacterial ResistanceSpectinomycinAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
C321.∆A ompT- rne- endA- lon-
Bacterial Strain#182775PurposeRecoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA, lon inactivatedDepositorBacterial ResistanceAmpicillinAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAL950
Plasmid#119748Purposeprecursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to CysteineDepositorInsertprecusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
UseTagsnoneExpressionBacterialMutationInternal NdeI site removed and mutations Alanine …PromoterT7Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR59.4
Plasmid#63175PurposeExpresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoterDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSR62
Plasmid#125076PurposeExpression of ompR under PLtetO-1, output promoter PompF146DepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQZ083
Plasmid#106417PurposeExpress Complexin-1 fragment (1-83) in E. coliDepositorInsertcomplexin-1 fragment 1-83 (Cplx1 Synthetic, Rat)
UseTagsGST tagExpressionBacterialMutationFragment 1-83, Codon optimizationPromoterT7Available sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV Flag WT-PTEN
Plasmid#22231DepositorInsertPTEN (PTEN Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mCherry-Nanobody
Plasmid#70696PurposeExpresses GST-tagged anti-mCherry Nanobody in E. coli cells.DepositorInsertmCherry-Nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2016AvailabilityAcademic Institutions and Nonprofits only