Skip to main content
Addgene
Showing: 781 - 800 of 1013 results

  1. pLenti-CaMKIIa-mTagBFP2

    Plasmid
    #87254
    Purpose
    Lentiviral fluorescent reporter using the neuronal cell type-specific promoter of the CaM Kinase II alpha gene.
    Depositor
    James Ellis
    Insert
    mTagBFP2
    Use
    Lentiviral
    Tags
    6xHis
    Expression
    Mutation
    Promoter
    CaMKIIa
    Available since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    87254-plasmid-map-sequence-id-174000

    Enlarge

  2. TRUPATH Triple GaoA

    Plasmid
    #196051
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoA-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196051-plasmid-map-sequence-id-385865

    Enlarge

  3. TRUPATH Triple GaoB

    Plasmid
    #196052
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoB-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196052-plasmid-map-sequence-id-387470

    Enlarge

  7. GABA(A) receptor subunit a1SE

    Plasmid
    #49168
    Purpose
    pHluorin-tagged GABA A receptor subunit (alpha 1) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Tija Jacob, Stephen Moss
    Article
    Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do
    Insert
    GABA(A) receptor subunit alpha-1 (Gabra1 Mouse)
    Use
    Tags
    myc and pHGFP (pHIuorin)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49168-plasmid-map-sequence-id-72484

    Enlarge

  8. Anti-Alpha-Tubulin [F2C] (Recombinant Antibody)

    Antibody
    #196559-rAb
    Purpose
    Anti-Alpha-Tubulin (Human) recombinant mouse monoclonal antibody
    Depositor
    Melina Fan
    Recommended Applications
    Immunocytochemistry
    Reactivity
    Human
    Source Species
    Mouse
    Isotype
    IgG2a
    Trial Size
    Available to purchase
    Available since
    March 10, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    Immunocytochemistry image by Addgene

  12. pKG204

    Plasmid
    #63078
    Purpose
    Retroviral expression of EGFP-TEV-S-tag HP1 alpha for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    HP1 alpha (CBX5 Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    V151I mutation compared to GenBank reference sequ…
    Promoter
    Available since
    Jan. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63078-plasmid-map-sequence-id-134726

    Enlarge

  15. pCAT-YFP-TetR

    Plasmid
    #59018
    Purpose
    Expresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondii
    Depositor
    David Sibley
    Article
    Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537
    Insert
    YFP-TetR
    Use
    Toxoplasma expression
    Tags
    Expression
    Mutation
    Promoter
    alpha-tubulin
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59018-plasmid-map-sequence-id-137741

    Enlarge

  17. pCAG-2dV-Camui (wt)

    Plasmid
    #220366
    Purpose
    Improved FLIM sensor for CaMK2-alpha activation (mammalian expression, cytosol)
    Depositor
    Ryohei Yasuda
    Article
    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-
    Insert
    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(wt)-mEGFP(A206K) (Camk2a Rat, Synthetic)
    Use
    Tags
    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    220366-plasmid-map-sequence-id-434512

    Enlarge

Showing: 781 - 800 of 1013 results