Addgene

We narrowed to 3,077 results for: zebrafish

Showing: 61 - 80 of 3077 results

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Tol2_B-act_dCas9-Vp64-T2A-Citrine

Plasmid

#200248

Purpose

Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

dCas9-Vp64-T2A-Citrine

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-mScarlet-MCS-polyA

Plasmid

#174023

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

mScarlet

Use

Zebrafish knock-in tagging

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-eGFP-MCS-polyA

Plasmid

#174024

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

eGFP

Use

Zebrafish knock-in tagging

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-eGFP_Y40N-MCS-polyA

Plasmid

#174025

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

eGFP

Use

Zebrafish knock-in tagging

Tags

Expression

Mutation

Y40N

Promoter

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-p2A-QF2-MCS-polyA

Plasmid

#174026

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-QF2

Use

Zebrafish knock-in tagging

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-p2A-tdTomato-polyA

Plasmid

#174027

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-tdTomato

Use

Zebrafish knock-in tagging

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-p2A-eGFP-MCS-polyA

Plasmid

#174028

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-eGFP

Use

Zebrafish knock-in tagging

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-p2A-mScarlet-MCS-polyA

Plasmid

#174029

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-mScarlet

Use

Zebrafish knock-in tagging

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-p2A-Venus-PEST-MCS-polyA

Plasmid

#174030

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-Venus-PEST

Use

Zebrafish knock-in tagging

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pHD157

Plasmid

#84033

Purpose

Vector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the fabp10 promoter and Venus under the crystalin promoter in zebrafish

Depositor

Didier Stainier, Daniel Hesselson

Article

Ni et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Inserts

Cre
Venus

Use

Cre/Lox; Zebrafish transgenesis

Tags

mCherry

Expression

Mutation

Promoter

Crystalin and fabp10

Availability

Academic Institutions and Nonprofits only

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pKE13-ISceI-fabp10a:CreERT2

Plasmid

#198242

Purpose

For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter driving tamoxifen-inducible Cre recombinase

Depositor

Didier Stainier

Article

Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.

Inserts

Zebrafish fabp10a promoter:Cre recombinase (Cre) fused to a mutant estrogen ligand-binding domain (ERT2), polyA (fabp10a Zebrafish)
Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)

Use

Zebrafish transgenesis

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Cas9-pA-Cry-GFP

Plasmid

#168239

Purpose

"neutrophil specific cas9 expression in zebrafish with green lens"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Cas9

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCS2-NfsB.zf1

Plasmid

#61387

Purpose

mRNA synthesis of bacterial nitroreductase NfsB codon modified for zebrafish

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

nfsB.zf1

Use

Zebrafish expression

Tags

Expression

Mutation

E28G

Promoter

sp6

Availability

Academic Institutions and Nonprofits only

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pCS2-Tol1.zf1

Plasmid

#61388

Purpose

mRNA synthesis of tol1 transposase codon modified for zebrafish

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

tol1.zf1

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

sp6

Availability

Academic Institutions and Nonprofits only

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pCS2-Cer.zf1

Plasmid

#61389

Purpose

mRNA synthesis of cerulean codon modified for zebrafish

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

Cer.zf1

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

sp6

Availability

Academic Institutions and Nonprofits only

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pCS2-TagRFPT.zf1

Plasmid

#61390

Purpose

mRNA synthesis of TagRFPT codon modified for zebrafish

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

TagRFPT.zf1

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

sp6

Availability

Academic Institutions and Nonprofits only

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pCS2-Cre.zf1

Plasmid

#61391

Purpose

mRNA synthesis of Cre recombinase codon modified for zebrafish

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

Cre.zf1

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

sp6

Availability

Academic Institutions and Nonprofits only

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pCS2-Gal4ff.zf1

Plasmid

#61392

Purpose

mRNA synthesis of Gal4ff codon modified for zebrafish

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

Gal4ff.zf1

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

sp6

Availability

Academic Institutions and Nonprofits only

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punc503GFP

Plasmid

#64021

Purpose

503-bp promoter of zebrafish unc-45b drives muscle-specific GFP expression

Depositor

Joachim Berger, Peter Currie

Article

Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

Insert

unc-45b: GFP (unc45b Zebrafish)

Use

Gateway

Tags

Expression

Mutation

Promoter

503-bp promoter of zebrafish unc-45b

Availability

Academic Institutions and Nonprofits only

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KI-eab2-AA

Plasmid

#182157

Purpose

Knockin plasmid with homologous arm for making zMADM-AA zebrafish line

Depositor

Hui Zong

Article

Xu et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

Homologous arm

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

eab2

Availability

Academic Institutions and Nonprofits only

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