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Plasmid#182405PurposeBacterial expression of Vesicle Nucleating peptide15-stefinA fusionDepositorInsertVNp15-stefin (CSTA Human)
UseTagsVesicle Nucleating peptide 15 (VNp15)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
p413TEF1-ATR1(pYL573)
Plasmid#178288PurposeExpresses NADPH-cytochrome P450 reductase 1 from A. thaliana (ATR1) in Saccharomyces cerevisiae. Centromeric HIS, PTEF1-ATR1-TCYC1DepositorInsertATR1
UseTagsExpressionYeastMutationPromoterTEF1Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp6-StefinA
Plasmid#182404PurposeBacterial expression of Vesicle Nucleating peptide6-stefinA fusionDepositorInsertVNp6-stefin (CSTA Human)
UseTagsVesicle Nucleating peptide 6 (VNp6)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPotef-cdc3CB16-NatR
Plasmid#86465PurposeExpresses an N-terminal Cdc3-mCherry fusion under the control of the constitutive Potef promotor. 16boxB stem-loops are integrated into the 3'UTR to visualize the mRNA.DepositorInsertCdc3
UseUstilago maydis expressionTagsmCherryExpressionBacterialMutationPromoterPotefAvailable sinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-StefinA
Plasmid#182403PurposeBacterial expression of Vesicle Nucleating peptide-stefinA fusionDepositorInsertVNp-stefin (CSTA Human)
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS426 TEF mCherry
Plasmid#129433Purposeexpress mcherryDepositorInsertmcherry
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMC6-TEFkFLP(LYS2)
Plasmid#49458Purposeoverespression of Flp step-arrest mutant in yeast, LYS2 selectionDepositorInsertFlp(H305L) (flp Budding Yeast)
UseTagsExpressionYeastMutationH305L, ‘step-arrest’ mutant,PromoterTEF1Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B8-TEF
Plasmid#44374DepositorInsertUAS1B8-TEF promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-TEFAvailable sinceApril 17, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
p413TEF-Sir2-H364Y
Plasmid#51743Purposeoverexpression of Sir2 in S. cerevisiae (H364Y mutant), HIS3 markerDepositorInsertSir2 (SIR2 Budding Yeast)
UseTagsExpressionYeastMutationH364YPromoterTEF1Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
p413TEF-Sir2-P394L
Plasmid#51744Purposeoverexpression of Sir2 in S. cerevisiae (P394L mutant), HIS3 markerDepositorInsertSir2 (SIR2 Budding Yeast)
UseTagsExpressionYeastMutationP394LPromoterTEF1Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-GFP
Plasmid#207018PurposeOver-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).DepositorInsertGFP
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYP-ptef1-GFP
Plasmid#199990PurposeYeast/E coli shuttle vector expressing GFP from tef1 promoterDepositorInsertGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_mNeongreen-StefinA
Plasmid#182418PurposeBacterial expression of mNeongreen-stefinA fusionDepositorInsertmNeongreen-stefin (CSTA Human)
UseTagsmNeongreenExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
p414-TEF1p-Cas9-CYC1t
Plasmid#43802DepositorArticleInsertHuman Optimized S. pyogenes Cas9
UseCRISPRTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B8-TEF(136)
Plasmid#44380DepositorInsertUAS1B8-TEF(136) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-TEF(136)Available sinceApril 17, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
Plasmid#83946PurposeCas9-carrying vector with kanMX markerDepositorInsertCas9
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
p415 TEF roGFP2‐Tsa2ΔCR
Plasmid#83238PurposeThe genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.DepositorInsertroGFP2-Tsa2dCr
UseTagsroGFP2 fusion with Tsa2dCrExpressionYeastMutationPromoterTEFAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B16-TEF(504)
Plasmid#44371DepositorInsertUAS1B16-TEF(504) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B16-TEF(504)Available sinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
Plasmid#78231PurposeExpresses Cas9 protein, under the control of the TEF1 promoter, and CYC terminator. KanMX resistanceDepositorInsertCas9
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pTEF1p-CYC1t
Plasmid#62190PurposeExpresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionYeastMutationhuman codon-optimized, D10A, H840APromoterTEF1 promoterAvailable sinceFeb. 24, 2015AvailabilityAcademic Institutions and Nonprofits only