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Plasmid#59373PurposeInducible expression of OleD (DQ195536) under T7 promoterDepositorInsertOleD
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-NEMOL329P
Plasmid#11969DepositorInsertNEMOL329P (IKBKG Human)
UseTagsHisExpressionBacterialMutationLeucine329 to ProlinePromoterAvailable sinceJuly 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-C
Plasmid#213496PurposeEncodes Node C of the three-node Turing circuitDepositorInsertslambda cI 5G6GP
mCherry
UseSynthetic BiologyTagsASV tag and LVA tagExpressionMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-hMICU1EF
Plasmid#199180PurposeThis plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.DepositorInsertMICU1 (MICU1 Human)
UseTagsHis6 and MBP, Thrombin site, TEV siteExpressionMutationD421A, E432K, F433W, F453WPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-PF2050
Plasmid#100721PurposeExpression of Pyrococcus furiosus PF2050DepositorInsertPF2050
UseTagsExpressionBacterialMutationPromoterT7 promotorAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-Gm1
Plasmid#53528PurposeExpresses a plant arginyl-tRNA synthetase in E.coliDepositorInsertarginyl-tRNA synthetase
UseTagsHis Tag and ThioredoxinExpressionBacterialMutationPromoterT7Available sinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-Cb2
Plasmid#53529PurposeExpresses an insect mitochondrial arginyl-tRNA synthetase in E.coliDepositorInsertmitochondrial arginyl-tRNA synthetase
UseTagsHis Tag and ThioredoxinExpressionBacterialMutationchanged Ser4 to Ala4 to permit NcoI cloningPromoterT7Available sinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-Ce25
Plasmid#51296PurposeExpresses a plant arginyl-tRNA synthetase in E.coliDepositorInsertarginyl-tRNA synthetase
UseTagsHis Tag and ThioredoxinExpressionBacterialMutationGTT (Val) insertion after initiation codon to per…PromoterT7Available sinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-UNC60B
Plasmid#48740PurposeBacterial expression of UNC-60B (a C. elegans ADF/cofilin)DepositorInsertC. elegans UNC-60B cDNA (unc-60 Nematode)
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-UNC60A
Plasmid#48739PurposeBacterial expression of UNC-60A (a C. elegans ADF/cofilin)DepositorInsertC. elegans UNC-60A cDNA (unc-60 Nematode)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-MRPL15
Plasmid#31345DepositorInsertmitochondrial ribosomal protein L15 mature form (MRPL15 Human)
UseTagsHisExpressionBacterialMutationHas the amino acids in the predicted mature form …PromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-sCS4T
Plasmid#28249DepositorInsertsingle chain Cse4-Scm3-H4 with Thrombin site
UseTagsHisExpressionBacterialMutationA thrombin cutting site between Cse4 and Scm3PromoterAvailable sinceJuly 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-sC4T
Plasmid#28251DepositorInsertsingle chain Cse4-H4 with Thrombin site
UseTagsHisExpressionBacterialMutationA thrombin cutting site between Cse4 and H4PromoterAvailable sinceJuly 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pET-17b_K490clm_E215C
Plasmid#169995PurposeKinesin KIF5B K490 cysteine light mutant with E215C mutation for single cysteine labelingDepositorInsertkinesin family member 5B, isoform CRA_a
UseTags6xHISExpressionBacterialMutationC7S, C65A, C168A, C174S, E215C, C294A, C330S, C42…PromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b
Plasmid#40571DepositorInsertAurora B FRET-sensor N-terminally fused to histone 2B
UseTagsCyPet and YPetExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-Sfp
Plasmid#159617PurposeExpression plasmid for Sfp phosphopantetheinyl transferaseDepositorInsertSfp
UseTags6HISExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-NDH6
Plasmid#105503PurposeExpression of an N-terminally truncated form of Drosophila topoisomerase I with a C-terminal 6His tagDepositorInsertNDH6
UseTags6xHisExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-21a_3xNLS_SpCas9_protein_expression
Plasmid#114365PurposeProtein expression plasmid for 3xNLS SpCas9 in pET-21a backboneDepositorInsert3xNLS_SpCas9
UseCRISPRTagsNLSExpressionBacterialMutationPromoterT7Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-GST
Plasmid#42049DepositorInsertGST
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only