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Plasmid#51296PurposeExpresses a plant arginyl-tRNA synthetase in E.coliDepositorInsertarginyl-tRNA synthetase
UseTagsHis Tag and ThioredoxinExpressionBacterialMutationGTT (Val) insertion after initiation codon to per…PromoterT7Available sinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-UNC60B
Plasmid#48740PurposeBacterial expression of UNC-60B (a C. elegans ADF/cofilin)DepositorInsertC. elegans UNC-60B cDNA (unc-60 Nematode)
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-UNC60A
Plasmid#48739PurposeBacterial expression of UNC-60A (a C. elegans ADF/cofilin)DepositorInsertC. elegans UNC-60A cDNA (unc-60 Nematode)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-MRPL15
Plasmid#31345DepositorInsertmitochondrial ribosomal protein L15 mature form (MRPL15 Human)
UseTagsHisExpressionBacterialMutationHas the amino acids in the predicted mature form …PromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-sCS4T
Plasmid#28249DepositorInsertsingle chain Cse4-Scm3-H4 with Thrombin site
UseTagsHisExpressionBacterialMutationA thrombin cutting site between Cse4 and Scm3PromoterAvailable sinceJuly 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-sC4T
Plasmid#28251DepositorInsertsingle chain Cse4-H4 with Thrombin site
UseTagsHisExpressionBacterialMutationA thrombin cutting site between Cse4 and H4PromoterAvailable sinceJuly 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pET-17b_K490clm_E215C
Plasmid#169995PurposeKinesin KIF5B K490 cysteine light mutant with E215C mutation for single cysteine labelingDepositorInsertkinesin family member 5B, isoform CRA_a
UseTags6xHISExpressionBacterialMutationC7S, C65A, C168A, C174S, E215C, C294A, C330S, C42…PromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pETM33_EIF4E_eiF4E
Plasmid#178474PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertEIF4E_eiF4E (EIF4E Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_3
Plasmid#78200PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,I76G,A197C)
UseTagsExpressionBacterialMutationA17C,I76G,A197C (please see depositor comment bel…PromoterT7Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_1
Plasmid#78198PurposeExpression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A197C)
UseTagsExpressionBacterialMutationA197C (please see depositors comment below)PromoterT7Available sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_2
Plasmid#78199PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,A197C)
UseTagsExpressionBacterialMutationA17C; A197C (please see depositor comment below)PromoterT7Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGreen_GUS_competitor
Plasmid#55208PurposeGUS-based microRNA target competitor used for testing function as target mimicryDepositorInsertbeta-glucuronidase
UsePlant expressionTagsExpressionMutationPromoterAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETM6
Plasmid#49795PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneUseTagsS-tagExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.b201
Plasmid#131404PurposeE. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albuminDepositorInsertNanobody b201
UseTags6xHis-tag and pelB leaderExpressionBacterialMutationPromoterT7Available sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15p65
Plasmid#27661DepositorInsertTetrahymena telomerase holoenzyme p65
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET16B.Pfu
Plasmid#12509PurposeBacterial expression of Pfu DNA polymeraseDepositorInsertPfu DNA polymerase
UseTagsExpressionBacterialMutationY218H, Q462R, F533L, K559I and V683A (mutations w…PromoterAvailable sinceSept. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pETcon(-)
Plasmid#41522DepositorTypeEmpty backboneUseTags3x(G4S) Linker, G3S Linker, HA, Myc Tag, and Stuf…ExpressionYeastMutationPromoterGAL1Available sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET11phiC31poly(A)
Plasmid#18942DepositorInsertphiC31 integrase (int Streptomyces phage phiC31)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28a_CnCas12f1
Plasmid#204996PurposeCnCas12f1 heterologous expression plasmidDepositorInsertCnCas12f1
UseCRISPRTagsExpressionMutationPromoterT7Available sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only