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Showing: 61 - 80 of 257 results

  4. pMal-mRuby3

    Plasmid
    #209348
    Purpose
    Protein expression vector with a TEV cleavable N-terminal 6xHis-MBP and a HRV 3C cleavable C-terminal mRuby3-StepTagII
    Depositor
    Lucia Strader
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    209348-plasmid-map-sequence-id-419546

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  5. pKK-BI16-ORF1-3C-mRuby3_ORF2-TEV-mClover3

    Plasmid
    #105802
    Purpose
    Concomitant expression of two proteins. One protein expressed with mRuby3, cleavable by 3C; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-mRuby3; ORF2: TEV-mClover3
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    105802-plasmid-map-sequence-id-203396

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  8. pAAV-hSyn-DIO-jGCamp7b-P2A-GSG-mRuby3

    Plasmid
    #130900
    Purpose
    bicistronic plasmid for dual-color neuronal calcium and morphology imaging
    Depositor
    Simon Wiegert
    Insert
    jGCaMP7b-P2A-mRuby3
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Availability
    Academic Institutions and Nonprofits only
    130900-plasmid-map-sequence-id-256581

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  16. pAAV_hSyn_GCaMP6m_sWPRE_spA_mDLX_nls_mRuby3_ssWPRE_spA

    Plasmid
    #205281
    Purpose
    CaMP used to measuring calcium changes in neurons, while nls-mRuby is employed to specifically identify cortical GABAergic neurons. This dual labeling approach allows for the simultaneous observation of neuronal activity using GCaMP and the identification of a specific subset of neurons using nls-mRuby, focusing specifically on cortical GABAergic neurons.
    Depositor
    Matthias Prigge
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    205281-plasmid-map-sequence-id-412571

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  18. AAV_CWSL.nEF.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3

    Plasmid
    #107736
    Purpose
    In the presence of Cre, can be used to express both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.
    Depositor
    Rylan Larsen
    Insert
    synaptophysin-GCaMP6s, mRuby3
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    nEF
    Availability
    Academic Institutions and Nonprofits only
    107736-plasmid-map-sequence-id-207278

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  19. AAV_CWSL.hSyn.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3

    Plasmid
    #107737
    Purpose
    In the presence of Cre, drives neuron-specific expression of both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.
    Depositor
    Rylan Larsen
    Insert
    synaptophysin-GCaMP6s, mRuby3
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Availability
    Academic Institutions and Nonprofits only
    107737-plasmid-map-sequence-id-207281

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Showing: 61 - 80 of 257 results