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Plasmid#183964PurposeCAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroRDepositorInsertHyperdCas12a
UseCRISPRTagsHA, Hygromycin Resistance, and mCherryExpressionMammalianMutationD156R, E292R, D235R, D350RPromoterCAGAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10873
Plasmid#183961PurposeU6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPRDepositorInsertsHyperdCas12a
poly-crRNA array targeting Sox2-Klf4-Oct4
UseCRISPRTagsHAExpressionMammalianMutationD832A, D156R, E292R, D235R, D350RPromoterCAG and U6Available sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL004
Plasmid#215336PurposeamyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank-Hy promoter)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterhyper-pSpankAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Tubulin K40Q
Plasmid#105302PurposeTo study effects of acetyl-mimetic (hyper-acetylated) tubulinDepositorInsertalpha tubulin (TUBA1B Human)
UseTagsEGFPExpressionMammalianMutationK40QPromoterAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC1-PIP-SHOW
Plasmid#42212PurposeGenetically encoded fluorescent indicator for simultaneous PIP3 and hydrogen peroxide detection.DepositorInsertPIP-SHOW
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMXs-EGFP-Rheb-HVR HRas-IP
Plasmid#13833DepositorInsertRheb-HVR HRas (Rheb Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationN-terminal of Rheb and hyper variable region (HVR…PromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SypHer2-DAAO-NES
Plasmid#119184PurposeFusion of the pH-sensitive fluorescent biosensor SypHer2 with D-amino acid oxidase; excluded from nucleus. CMV promoter. Can serve as a pH control for HyPer.DepositorInsertSypHer2-DAAO-NES
UseAAVTagsNESExpressionMammalianMutationPromoterCMVAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXcX-PRS-EGFP
Plasmid#25865DepositorInsertPRS driven enhanced green fluorescent protein
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
p09008_AF1-4+GFP5-7
Plasmid#173715PurposePlasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.DepositorInsertshybrid GFP
mKate2
UseE. coli cell free protein synthesis extractsTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
TaRGET-ABE-C3.1 ver4.1
Plasmid#188251PurposeHypercompact adenine base editorsDepositorInsertTaRGET-ABE3.1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-Hrp48-ADARcd-E488Q-V5
Plasmid#139685PurposeExpresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assaysDepositorInsertHrp48, ADAR (Adar Fly)
UseTagsV5ExpressionBacterial and InsectMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT-ADARcd-E488Q-V5
Plasmid#139686PurposeExpresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5DepositorInsertAdenosine deaminase acting on RNA (Adar Fly)
UseTagsV5ExpressionBacterial and InsectMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
TaRGET-ABE-C3.1 ver4.0
Plasmid#188250PurposeHypercompact adenine base editorsDepositorInsertTaRGET-ABE3.1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSI2-ADA
Plasmid#166967PurposeExpress the fusion protein of human MSI2 and the hyperactive catalytic domain of Drosophila ADARDepositorInsertMSI2-ADA
UseRetroviralTagsMSI2-ADAExpressionMammalianMutationPromoterMSCVAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP
Plasmid#154786PurposeExpresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBEDepositorInsertAdenosine deaminse (Adar Synthetic, Fly)
UseLentiviralTagsGFP, V5, and mCherryExpressionMammalianMutationSynonymized serine to prevent autoediting of ADAR…PromoterUBCAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti-HA-mBec1 F121A
Plasmid#99506PurposeLentiviral expression of mutant Becn1 leading to hyperactive autophagyDepositorInsertBeclin 1 (Becn1 Mouse)
UseLentiviralTagsHAExpressionMutationF121APromoterCMVAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
R713-M52-303: CMV51p> Halotag-NRasHVR
Plasmid#159686PurposeMammalian protein expression of NRAS HVR (Hypervariable region)-Halotag fusionDepositorInsertHalotag-NRasHVR (NRAS Human)
UseTagsHalotagExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
AID-C12*-dCas9-BE4max
Plasmid#216731PurposeExpresses a BE4max base editing construct comprised of the hyperactive AID-C12* and dCas9 (Cas9 with D10A and H840A mutations) in mammalian cells.DepositorInsertAID-C12*-dCas9-BE4max (AICDA Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
AID-C12*-BE4max
Plasmid#216729PurposeExpresses a BE4max base editing construct comprised of the hyperactive AID-C12* and nCas9 (Cas9 with D10A mutation) in mammalian cells.DepositorInsertAID-C12*-BE4max (AICDA Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
R713-M50-303: CMV51p> Halotag-HRasHVR
Plasmid#159685PurposeMammalian protein expression of HRAS HVR (Hypervariable region)-Halotag fusionDepositorInsertHalotag-HRasHVR (HRAS Human)
UseTagsHalotagExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only