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Plasmid#236156PurposeFuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with eight IKZF1 binding sites upstreamDepositorInsertH2B (H2BC11 Human)
UseTagsEGFPExpressionMammalianMutationPromoterE1b minimal promoter with eight IKZF1 binding sit…Available sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
H2B-GFP-INCENP∆cen
Plasmid#184044PurposeExpresses H2B-GFP fused to INCENP∆cenDepositorInsertINCENP (INCENP Human)
UseTagsGFP and H2BExpressionMammalianMutationTruncated to 1-862PromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Zeo-H2BC11
Plasmid#207759PurposeDonor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorInsertH2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
3` CC: Hygro – H2B-GFP-N – loxP – mScarlet
Plasmid#219565Purpose3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosomeDepositorInsertHygroR_2A_H2B-GFP-N_loxP_mScarlet
UseUnspecifiedTagsExpressionMutationPromoterEF-1aAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-GFP
Plasmid#159654PurposeEmpty backbone for cloning gRNA sequences for DNA targeting. Contains an T2A linked H2B GFP nuclear reporter.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ421 - Pmyo-2::GFP::H2B (pharynx)
Plasmid#34876DepositorInsertPmyo-2 GFP H2B tbb-2 UTR
UseWorm targetingTagsExpressionMutationS65CPromotermyo-2Available sinceMarch 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCFJ420 - Peft-3::GFP::H2B (ubiquitous)
Plasmid#34877DepositorInsertPeft-3 GFP H2B tbb-2 UTR
UseWorm targetingTagsExpressionMutationS65CPromotereef-1A.1 (eft-3)Available sinceFeb. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
R26-H2B-EGFP HR donor vector
Plasmid#137925PurposeR0SA26 knock-in vector with homologous recombination-based methodDepositorInsertSA-H2B-EGFP-bpA with homology arms
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
Gata3-T2A-H2B-eGFP donor
Plasmid#113119PurposeDonor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Gata3 coding sequenceDepositorInsertGata3 donor region including homology arms and T2A-H2B-eGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pdgfra-T2A-H2B-eGFP donor
Plasmid#113120PurposeDonor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequenceDepositorInsertPdgfra donor region including homology arms and T2A-H2B-eGFP (Pdgfra Mouse, Synthetic)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-H2B-sfGFP-T2A-iCre
Plasmid#183351PurposeHistone H2B-sfGFP and iCre recombinaseDepositorInsertHistone H2B-sfGFP-T2A-iCre
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
H2B-GFP-AsLOV2-NES27 (pDN158)
Plasmid#72659PurposeConstitutively nuclear variant of a very strong, photocaged NES (AsLOV2-NES 27) for blocking endogenous, CRM-1-dependent nuclear exportDepositorInsertH2B-GFP-AsLOV2-NES27
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Puro-H2BC11-MMEJ
Plasmid#207760PurposeMMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorInsertH2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
EW408 (ZNF35tar-compact)x8-H2B-GFP
Plasmid#236129PurposeFuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with eight ZNF35 binding sites upstreamDepositorInsertH2B (H2BC11 Human)
UseTagsEGFPExpressionMammalianMutationPromoterE1b minimal promoter with eight ZNF35 binding sit…Available sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
SCN1Aprom(335-406)-H2B-GFP
Plasmid#236151PurposeFluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the SCN1a promoter region encompassing 335 to 406 bp upstream of its transcriptional start siteDepositorInsertH2B (H2BC11 Human)
UseTagsEGFPExpressionMammalianMutationPromoterE1b minimal promoter with the SCN1a promoter regi…Available sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-H2B.APEX-P2A-EGFP
Plasmid#182825PurposeCre-dependent expression of H2B-APEX fusionDepositorInsertH2B.APEX-P2A-EGFP
UseAAVTagsEGFP reporter (P2A)ExpressionMutationPromoterEF1aAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
SOX9-T2A-H2B-EGFP repair template
Plasmid#167972PurposeSOX9 reporter repair templateDepositorInsertSRY-Box Transcription Factor 9 (SOX9 Human)
UseCRISPR; Donor templateTagsH2B-EGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Neo-CAG-M2rtTA-H2BGFP (donor AB)
Plasmid#85798PurposedonorAB targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP expression systemDepositorInsertPPP1R12C (PPP1R12C Human)
UseHuman targetingTagsExpressionMutationhomology arms for knock-in into first intronPromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-loxP-H2B-sfGFP
Plasmid#136064PurposeH2B-sfGFP cre reporterDepositorInsertHIST1H2BB (H2BC3 Human)
UseCre/LoxTagssfGFPExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_H2B-HaloTag10-T2A-EGFP
Plasmid#175521PurposeNuclear expression of HaloTag10 in mammalian cellsDepositorInsertH2B-HaloTag10
UseTagsExpressionMammalianMutationHaloTag10 = HaloTag7-Q165WPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only