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Plasmid#74422PurposeExpress fluorescent calcium indicator GCaMP3fast in mammalian cellsDepositorInsertGCaMP3fast
UseTagsExpressionMammalianMutationW4Y D356APromoterCMVAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HT JS-Munc18c
Plasmid#135555PurposeExpress mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.DepositorInsertMunc18c
UseTags6xHis tag and TEV siteExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG328 td-iFAST
Plasmid#130811PurposeExpresses td-iFAST in mammalian cellsDepositorInserttd-iFAST
UseTagsmyc-tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-Nucleocapsid
Plasmid#179696PurposeExpresses H6 tagged SARS CoV-2 nucleocapsid in Sf9 cellsDepositorInsertSARS-CoV-2 Nucleocapsid (N protein) (N Severe acute respiratory syndrome coronavirus 2)
UseTagsHis6-TEV protease siteExpressionInsectMutationPromoterpolyhedrinAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
SPRR2A-pFastBac I
Plasmid#182460PurposeExpression His-tagged SPRR2A in insect cellsDepositorInsertsmall proline rich protein 2A (SPRR2A Human)
UseTags6xHisExpressionInsectMutationPromoterPolyhedrinAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-His10TEV
Plasmid#159427Purposeempty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sitesDepositorTypeEmpty backboneUseTagsTEV-protease-cleavable His10-tagExpressionInsectMutationPromoterpolyhedrinAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
mEos2-Fascin-C-10
Plasmid#57376PurposeLocalization: Actin Bundling Protein, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertFascin (FSCN1 Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEmerald-Fascin-N-10
Plasmid#54095PurposeLocalization: Actin Bundling Protein, Excitation: 487, Emission: 509DepositorInsertFascin (FSCN1 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac1 PIK3R1-Ni
Plasmid#17744DepositorInsertPIK3R1-Ni (PIK3R1 Human)
UseTagsExpressionInsectMutationPartial cDNAPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFastBac mPh1
Plasmid#1970DepositorInsertmPh1 (Phc1 Mouse)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFastBac K-M33
Plasmid#1986DepositorInsertM33 (Cbx2 Mouse)
UseTagsExpressionInsectMutationKozak sequence added before ATGPromoterAvailable sinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-HS-NLRC4Δcard
Plasmid#177276PurposePurification of mouse NLRC4 (90-1024) from insect cellsDepositorInsertNLRC4 (Nlrc4 Mouse)
UseTagsHIS-SUMO-PreScissionExpressionInsectMutationI586T, N761S, M885V, K915R and F972L- please see …PromoterpolyhedrinAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG330 td-FAST
Plasmid#130810PurposeExpresses td-FAST in mammalian cellsDepositorInserttd-FAST
UseTagsmyc-tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HT JS-Munc18b
Plasmid#135554PurposeExpress rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.DepositorInsertMunc18b (Stxbp2 Rat)
UseTags6xHis tag and TEV siteExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac FPC
Plasmid#1927DepositorInsertPolycomb (Pc Fly)
UseTagsflagExpressionInsectMutationPromoterAvailable sinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-pGC-A
Plasmid#186626PurposepFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)DepositorInsertNPR1 (NPR1 Human)
UseTagshemagglutinin (HA) signal peptide + His10-tag + T…ExpressionInsectMutationdeleted amino acids 1-32PromoterpolyhedringAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBacI/MDR3cysless
Plasmid#74400Purposeexpression of MDR3 in insect cellsDepositorInsertMDR3 (Abcb1a Mouse)
UseTags10HisExpressionInsectMutationall Cys to AlaPromoterPolyhedrinAvailable sinceMarch 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac K-Ring1A
Plasmid#1994DepositorInsertRing1A (Ring1 Mouse)
UseTagsExpressionInsectMutationKozak before start codonPromoterAvailable sinceMarch 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFastBac FPH
Plasmid#1925DepositorUseTagsFlagExpressionInsectMutationPromoterAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFastBac FPSC
Plasmid#1926DepositorInsertposterior sex combs (Psc Fly)
UseTagsflagExpressionInsectMutationPromoterAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only