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Showing: 61 - 80 of 430 results

  4. PET.SUMO eIF4G1 +MIC 1-200

    Plasmid
    #158792
    Purpose
    Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform including the microexon.
    Depositor
    Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1
    Insert
    eIF4G1 (with microexon) codon optimized
    Use
    Tags
    6xHIS and SUMO
    Expression
    Bacterial
    Mutation
    amino acids 1-200
    Promoter
    T7
    Available since
    Aug. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    158792-plasmid-map-sequence-id-309816

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  5. pT7-EGFP-C1-HseIF4E-F47AI79A_Z

    Plasmid
    #148136
    Purpose
    Mammalian Expression of HseIF4E-F47AI79A
    Depositor
    Elisa Izaurralde
    Insert
    HseIF4E-F47AI79A (EIF4E Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    148136-plasmid-map-sequence-id-390263

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  8. pcDNA5 rtTA BirA eIF4G

    Plasmid
    #158789
    Purpose
    pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 lacking the microexon for generation of N2A FlpIn cell lines for BioID
    Depositor
    Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1
    Insert
    eIF4G1
    Use
    Tags
    3xFlag and BirA*
    Expression
    Mammalian
    Mutation
    Promoter
    miniCMV
    Available since
    Aug. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    158789-plasmid-map-sequence-id-309804

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  10. R777-E056 Hs.EIF4EBP1-nostop

    Plasmid
    #70340
    Purpose
    Gateway ORF clone of human EIF4EBP1 [NM_004095.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    EIF4EBP1 (EIF4EBP1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70340-plasmid-map-sequence-id-125789

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  11. pOCC102-MBP-eIF4G-459

    Plasmid
    #219975
    Purpose
    eIF4G-459 protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G-459 (TIF4631 Budding Yeast)
    Use
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    459LL460 to 459AA460
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219975-plasmid-map-sequence-id-436889

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  12. pOCC240-MBP-eIF4A-mCherry

    Plasmid
    #219982
    Purpose
    eIF4A-mCherry protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4A-mCherry (TIF1 Budding Yeast)
    Use
    Tags
    MBP and mCherry-6xHis
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219982-plasmid-map-sequence-id-436882

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  13. pOCC102-MBP-eIF4G-SkA

    Plasmid
    #219977
    Purpose
    eIF4G-SkA protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G-SkA (TIF4631 Budding Yeast)
    Use
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    eIF4A binding domain of S. kudriavzevii
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219977-plasmid-map-sequence-id-436800

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  14. pOCC102-MBP-eIF4G-213

    Plasmid
    #219974
    Purpose
    eIF4G-213 protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G-213 (TIF4631 Budding Yeast)
    Use
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    213VKLR216 to 213AAAA216
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219974-plasmid-map-sequence-id-436799

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  15. pOCC121-MBP-monoGFP-eIF4G

    Plasmid
    #219972
    Purpose
    eIF4G protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G (TIF4631 Budding Yeast)
    Use
    Tags
    6xHis and MBP-monoGFP
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219972-plasmid-map-sequence-id-436768

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  16. pOCC102-MBP-eIF4G-RAR

    Plasmid
    #219973
    Purpose
    eIF4G-RAR protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G-RAR (TIF4631 Budding Yeast)
    Use
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    Truncation: amino acids 490 to 942
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219973-plasmid-map-sequence-id-436750

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Showing: 61 - 80 of 430 results