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Showing: 61 - 80 of 9652 results

  2. pCRISPR-Cas9/CD4-TK2

    Plasmid
    #104397
    Purpose
    The designed sgRNA cloned into this plasmid directs the specific DNA cleavage exerted by Cas9 nuclease in a region of exon 5 of the human TK2 gene. The plasmid also includes the Cas9 nuclease and CD4.
    Depositor
    Jeronimo Blanco
    Article
    Meca-Cortes et al Mol Ther Nucleic Acids. 2017 Sep 15;8:39
    Insert
    TK2 (TK2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    104397-plasmid-map-sequence-id-208338

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  3. LentiCRISPRv2-ACTB-C4

    Plasmid
    #229849
    Purpose
    Expresses wild-type Cas9 and gRNA for ACTB gene. The target sequence is changed from ACTB-C1 to improve the cut efficiency.
    Depositor
    Natsuko Chiba
    Insert
    guide RNA for ACTB gene
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    229849-plasmid-map-sequence-id-462154

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  13. pCRISPR3_BC2

    Plasmid
    #191857
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Kimberly Reynolds
    Article
    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    191857-plasmid-map-sequence-id-381604

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  14. pCRISPR3_BC6

    Plasmid
    #191861
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CCTAGT) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Kimberly Reynolds
    Article
    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    191861-plasmid-map-sequence-id-379165

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  15. pCRISPR3_BC5

    Plasmid
    #191860
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Kimberly Reynolds
    Article
    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    191860-plasmid-map-sequence-id-379164

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  16. pCRISPR3_BC4

    Plasmid
    #191859
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Kimberly Reynolds
    Article
    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    191859-plasmid-map-sequence-id-379162

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  17. pCRISPR3_BC3

    Plasmid
    #191858
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Kimberly Reynolds
    Article
    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    191858-plasmid-map-sequence-id-379160

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  18. pCRISPR3_BC1

    Plasmid
    #191856
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Kimberly Reynolds
    Article
    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    191856-plasmid-map-sequence-id-379154

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  20. pCRISPRa_VPR_yl

    Plasmid
    #107677
    Purpose
    CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Article
    Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:
    Inserts
    Codon optimized dCas9-VPR
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    May 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    107677-plasmid-map-sequence-id-209648

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Showing: 61 - 80 of 9652 results