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Plasmid#133421PurposeS. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).DepositorUseTagsExpressionBacterialMutationORF for Nip1 encodes aa 4-156 (not complete ORF),…PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only
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Kek1 (Lip1) clone #1
Plasmid#38750DepositorInsertKek1 (kek1 Fly)
UseTagsExpressionMutationPromoterAvailable sinceSept. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBS hTSP2 clone M1-B
Plasmid#12412DepositorInsertM1-B TSP2 (THBS2 Human)
UseTagsExpressionBacterialMutationFragment from Heparin Binding domain and Type 1 r…PromoterAvailable sinceOct. 23, 2006AvailabilityAcademic Institutions and Nonprofits only -
EasyCloneYALI- CRISPR/Cas9 Set
Plasmid Kit#1000000141PurposeContains plasmids for genome editing of Y. lipolytica via CRISPR/Cas9DepositorAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
EasyCloneYALI- Integrative Vector Set
Plasmid Kit#1000000140PurposeContains integrative plasmids suitable for (over-)expression of (heterologous) genes in Y. lipolytica strainsDepositorAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
EasyClone-MarkerFree Vector Set
Plasmid Kit#1000000098PurposeYeast (S. cerevisiae) vector toolkit for marker-less integration of genes into 11 predetermined EasyClone Chromosomal loci via CRISPR-Cas9DepositorAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
EasyClone 2.0 Yeast Toolkit
Plasmid Kit#1000000073PurposeIntegrative constructs for (over-) expression of (heterologous) genes in both laboratory and industrial yeast (S. cerevisiae) strains; Allow for selection in auxotrophic and prototrophic yeast strainsDepositorAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
The Expanded EasyClone-MarkerFree toolkit
Plasmid Kit#1000000190PurposeThe Expanded EasyClone-MarkerFree toolkit is a new set of 8 validated chromosomal loci for the stable integration of heterologous DNA into baker’s yeast Saccharomyces cerevisiae.DepositorAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 - TRC cloning vector
Plasmid#10878Purpose3rd generation transfer plasmidDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 LIC cloning vector (6A)
Plasmid#30124DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pORANGE Cloning template vector
Plasmid#131471PurposeCloning template form ORANGE method based knock-in constructsDepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
sgRNA(MS2) cloning backbone
Plasmid#61424PurposesgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2+mScarlet-C Cloning Vector
Plasmid#128147PurposeTo express a protein of interest fused to the C-terminus of mScarletDepositorTypeEmpty backboneUseTagsmScarlet fusionExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-APEX2-C Cloning Vector
Plasmid#128146PurposeTo express a protein of interest fused to the C-terminus of Flag-APEX2 for proximity labeling or TEMDepositorTypeEmpty backboneUseTagsFlag-APEX2ExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHKMC1: Empty sgRNA for Cloning
Plasmid#67720PurposeEmpty vector for cloning sgRNA. NotI and BamHI for cloning sgRNA. PU6 driven sgRNA.DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterPU6Available sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2+mNeonGreen-C Cloning Vector
Plasmid#128144PurposeTo express a protein of interest fused to the C-terminus of mNeonGreenDepositorTypeEmpty backboneUseTagsmNeonGreen fusionExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOC1 cloning template vector
Plasmid#183420PurposeCreON knock-in vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOC2 cloning template vector
Plasmid#183421PurposeCreOFF knock-in vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET LIC cloning vector (2A-T)
Plasmid#29665DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFastBac LIC cloning vector (4A)
Plasmid#30111DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only