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Plasmid#86556PurposeFor ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)DepositorInsertCas9
UseCRISPRTagsExpressionPlantMutationCas9 is codon optimized for C. reinhardtiiPromoterUBIQUITIN10Available sinceMay 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cas9-PCV
Plasmid#123644PurposeExpresses Cas9 with a C-terminal HUH-tag called PCV2 in E. coliDepositorInsertsUseTags6xHis and SUMOExpressionBacterialMutation2-116PromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
modRNAc0-Cas9
Plasmid#170180PurposeIVT template plasmid for making modified Cas9 RNADepositorInsertCas9
UseIvt template plasmidTagsExpressionMutationPromoterT7 promoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-Cas9
Plasmid#64237Purposeexpresses codon optimized Cas9 in zebrafishDepositorInsertcodon optimized Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAEL006511-Cas9
Plasmid#100580PurposeThe promoter of AAEL006511 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL006511-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectMutationPromoterAAEL006511 and Opie2Available sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
PCS2+ Cas9
Plasmid#122948PurposeFor in vitro transcription to produce Cas9 mRNA for microinjection into mouse embryosDepositorInsertCas9
UseSynthetic BiologyTagsExpressionMutationPromoterSP6Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAEL005635-Cas9
Plasmid#100608PurposeThe promoter of AAEL005635 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL005635-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectMutationPromoterAAEL005635 and Opie2Available sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAEL007097-Cas9
Plasmid#100705PurposeThe promoter of AAEL007097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL007097-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectMutationPromoterAAEL007097 and Opie2Available sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAEL003877-Cas9
Plasmid#100581PurposeThe promoter of AAEL003877 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL003877-hspcas9-GFP
Opie-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectMutationPromoterAAEL003877 and Opie2Available sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cas9 NGFR
Plasmid#155279PurposeLentiviral Cas9 expression with NGFR markerDepositorInsertspCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
SETMAR-Cas9
Plasmid#186701PurposePlasmid encoding SETMAR-Cas9 fusion under CMV promoterDepositorInsertSETMAR-Cas9
UseCRISPRTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cas9 CFP
Plasmid#155278PurposeLentiviral Cas9 expression with CFP markerDepositorInsertspCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAEL007584-Cas9
Plasmid#100706PurposeThe promoter of AAEL007584 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL007584-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectMutationPromoterAAEL007584 and Opie2Available sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pGWT35S-Cas9
Plasmid#186723PurposeTransient expression of Cas9 in plant cells.DepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant
Plasmid#101217PurposeBacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variantDepositorInsertSpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationK848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…PromoterT7Available sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLP774_dCas9-Spy-Snoop-Sunx5-Avi-Tag-BFP (dCas9-SSSavi-BFP)
Plasmid#211767PurposedCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), with BFP selectionDepositorInsertdCas9, Spy, Snoop, aGCN4, AviTags
UseTags3xHAExpressionMammalianMutationdCas9 D10A and H840APromoterpGK and pEF1aAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant
Plasmid#101230PurposeBacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variantDepositorInsertSpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, E60C, D273C, N692A, M694A, Q695A and…PromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC282 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant
Plasmid#101231PurposeBacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variantDepositorInsertSpCas9 variant C80S/C574S/S701C/S960C/N692A/M694A/Q695A/H698A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, S701C, S960C, N692A, M694A, Q695A an…PromoterT7Available sinceNov. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant
Plasmid#101229PurposeBacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variantDepositorInsertSpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A
UseTags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, S355C, S867C, N692A, M694A, Q695A an…PromoterT7Available sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only