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Showing: 61 - 80 of 709 results

  1. K560_AviN_coBirA

    Plasmid
    #129766
    Purpose
    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    Avi-tag GLNDIFEAQKIEWH and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Promoter
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129766-plasmid-map-sequence-id-263552

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  6. LEMD3_BirA_Nterm

    Plasmid
    #221518
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Lemd3
    Depositor
    Alessandro Ori
    Insert
    LEMD3 (LEMD3 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    221518-plasmid-map-sequence-id-439442

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  7. MAPK9_BirA_Nterm

    Plasmid
    #221521
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Mapk9
    Depositor
    Alessandro Ori
    Insert
    MAPK9 (MAPK9 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221521-plasmid-map-sequence-id-439409

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  8. LEMD3_BirA_Cterm

    Plasmid
    #221517
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Lemd3
    Depositor
    Alessandro Ori
    Insert
    LEMD3 (LEMD3 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221517-plasmid-map-sequence-id-439380

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  9. MAPK8_BirA_Cterm

    Plasmid
    #221519
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Mapk8
    Depositor
    Alessandro Ori
    Insert
    MAPK8 (MAPK8 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    221519-plasmid-map-sequence-id-439379

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  10. PSMD3_BirA_Nterm

    Plasmid
    #221526
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Psmd3
    Depositor
    Alessandro Ori
    Insert
    PSMD3 (PSMD3 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221526-plasmid-map-sequence-id-439227

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  11. PSMC3_BirA_Nterm

    Plasmid
    #221524
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Psmc3
    Depositor
    Alessandro Ori
    Insert
    PSMC3 (PSMC3 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221524-plasmid-map-sequence-id-439226

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  12. SYNJ1_BirA_Nterm

    Plasmid
    #221523
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Synj1
    Depositor
    Alessandro Ori
    Insert
    SYNJ1 (SYNJ1 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221523-plasmid-map-sequence-id-439225

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  13. SYNJ1_BirA_Cterm

    Plasmid
    #221522
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Synj1
    Depositor
    Alessandro Ori
    Insert
    SYNJ1 (SYNJ1 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221522-plasmid-map-sequence-id-439224

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  14. PSMA4_BirA_Cterm

    Plasmid
    #221513
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Psma4
    Depositor
    Alessandro Ori
    Insert
    PSMA4 (PSMA4 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221513-plasmid-map-sequence-id-439223

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  15. MAPK9_BirA_Cterm

    Plasmid
    #221520
    Purpose
    Flag-tagged biotinylation enzyme for co-expression of Mapk9
    Depositor
    Alessandro Ori
    Insert
    MAPK9 (MAPK9 Human)
    Use
    Tags
    BirA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221520-plasmid-map-sequence-id-439222

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  18. pMT-sox10-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80063
    Purpose
    Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Sox10 promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80063-plasmid-map-sequence-id-417621

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  19. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

    Plasmid
    #79887
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79887-plasmid-map-sequence-id-402553

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  20. pMT-ubb-NLS-BirA-2a-mCherry

    Plasmid
    #79886
    Purpose
    Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79886-plasmid-map-sequence-id-150062

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Showing: 61 - 80 of 709 results