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Plasmid#162708PurposeSecond CCM operon cloned from H. neapolitanus; pFA expressing the remaining 11 genes in the H. neapolitanus CCM clusterDepositorInsertSecond CCM operon cloned from H. neapolitanus
UseTagsExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
p1A CbbL-
Plasmid#162693Purposep1A negative control expressing inactive CbbLS (carbamylated K194M mutation) and PrkDepositorInsertH. neapolitanus CbbLS and S. elongatus Prk
UseTagsN terminal 6x His tag on PRKExpressionBacterialMutationCbbL K194M (inactive rubisco)PromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFE-Rrub-FII-prk
Plasmid#162695PurposeExpresses R. rubrum Form II rubisco and S. elongatus PrkDepositorInsertsRubrum Form II rubisco
S. elongatus PRK
UseTagsExpressionBacterialMutationPromoterPLtet0-1 promoter and under same promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCB’ CsoS2 ΔNTD
Plasmid#162705PurposeCarboxysome operon with truncated CsoS2 (deletion of amino acids 2-256), prk; Deletion of rubisco-binding N-terminus of CsoS2DepositorInsertpHnCB10 derived carboxysome operon, prk
UseTagsExpressionBacterialMutationDeletion of residues 2-256 of CsoS2, Y131DPromoterPLtet0-1 promoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCB’ CbbL Y72R
Plasmid#162707PurposeCarboxysome operon with point mutation to CbbL, prk; Substitution of CsoS2-binding tyrosine with arginine prevents rubisco encapsulation creating rubisco-less carboxysomesDepositorInsertpHnCB10 derived carboxysome operon, prk
UseTagsExpressionBacterialMutationCbbL Y72R; Substitution of CsoS2-binding tyrosine…PromoterPLtet0-1 promoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCCM’ CbbQ-
Plasmid#162711PurposeSecond CCM operon with inactive CbbQ (K46A, E107Q) rubisco activaseDepositorInsertSecond CCM operon cloned from H. neapolitanus
UseTagsExpressionBacterialMutationInactivation of CbbQ subunit of CbbOQ rubisco act…PromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCCM’ ΔacRAF
Plasmid#162710PurposeSecond CCM operon lacking acRAF; Deletion of putative rubisco chaperone, acRAFDepositorInsertSecond CCM operon cloned from H. neapolitanus
UseTagsExpressionBacterialMutationpCCM' with a deletion of putative rubisco ch…PromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
p1A Prk-
Plasmid#162694Purposep1A negative control expressing CbbLS and inactive Prk (Prk K20M S21A)DepositorInsertH. neapolitanus CbbLS and S. elongatus Prk
UseTagsN terminal 6x His tag on PRKExpressionBacterialMutationPrk inactive (K20M S21A native protein, this map …PromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-3'3xFlag
Plasmid#208616PurposeEmpty vector for mammalian expression, insert 3xFlag tag using BamHI andAflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with 3xFlagDepositorTypeEmpty backboneUseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-3'TwStrep
Plasmid#208618PurposeEmpty vector for mammalian expression, insert Twin Strep tag using BamHI and AflII site of pcDNA3.1 (-), both site retained, BamHI in-frame with Twin Strep tagDepositorTypeEmpty backboneUseTagsTwin StrepExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-H12TEV
Plasmid#208635Purposefor E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.DepositorTypeEmpty backboneUseTags6xhisExpressionBacterialMutationPromoterT7Available sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-5'TwStrep
Plasmid#208620PurposeEmpty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.DepositorTypeEmpty backboneUseTagsTwin StrepExpressionBacterialMutationPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBLO 4.01
Plasmid#121495Purposeengineered Mu transposonDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterp15AAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBLO44.5_PVYpro
Plasmid#121634PurposeCMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterchicken beta actin promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBLO44.3_TuMVpro
Plasmid#121632PurposeCMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterchicken beta actin promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBLO44.4_PPVpro
Plasmid#121633PurposeCMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterchicken beta actin promoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBLO44.7_WNVpro
Plasmid#121638PurposeCMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterchicken beta actin promoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBLO44.6_ZIKVpro
Plasmid#121635PurposeCMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterchicken beta actin promoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCKanR-Mu-BsaI
Plasmid#79769PurposeTransposon-propagation plasmid for Mu-BsaI modified transposonDepositorInsertMu-BsaI
UseTagsExpressionMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pATT-Dest
Plasmid#79770PurposeStaging plasmid for use in DIP transpositionDepositorTypeEmpty backboneUseSynthetic Biology; Staging plasmid for transposit…TagsExpressionMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only