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Showing: 61 - 80 of 894 results

  1. pTH1-SpSrtA

    Plasmid
    #64987
    Purpose
    Expresses the wild type of Streptococcus pyogenes sortase A in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955
    Insert
    S. pyogenes sortase A
    Use
    Tags
    His6 tag, Mxe GyrA intein, and chitin-binding pro…
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-81
    Promoter
    T7 promoter
    Available since
    June 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    64987-plasmid-map-sequence-id-109847

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  3. pCri-H6-TEV-GRAB

    Plasmid
    #128498
    Purpose
    Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.
    Depositor
    F Xavier Gomis-Ruth
    Article
    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.
    Insert
    GRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)
    Use
    Tags
    His tag (6x)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    128498-plasmid-map-sequence-id-251962

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  4. FU_tetO_dCas9-VP192-T2A-EGFP

    Plasmid
    #121173
    Purpose
    Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.
    Depositor
    Timo Otonkoski
    Insert
    dCas9-VP192-T2A-EGFP
    Use
    CRISPR and Lentiviral; Inducible by doxycycline
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    121173-plasmid-map-sequence-id-234995

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  5. pET15b-ACPs (from S. pneumonia)

    Plasmid
    #63687
    Purpose
    Inducible expression of Acyl Carrier Protein Synthase (ACPs) from S. pneumoniae
    Depositor
    Michael Johnson
    Insert
    acpS
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1

    Plasmid
    #133340
    Purpose
    for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentus
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    sgRNA_gcrA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    constitutive
    Available since
    Jan. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    133340-plasmid-map-sequence-id-269542

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  18. CMV-dCas9_puroR

    Plasmid
    #219825
    Purpose
    CMV-driven expression of dCas9 with puromycin resistance (Adapted from plasmid #68416)
    Depositor
    Philipp Maass
    Insert
    dCas9
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    CMV
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219825-plasmid-map-sequence-id-441124

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  20. pPEPY-PF6-lacI

    Plasmid
    #85589
    Purpose
    Work as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniae
    Depositor
    Jan-Willem Veening
    Article
    Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do
    Insert
    lacI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PF6
    Available since
    Feb. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    85589-plasmid-map-sequence-id-172946

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Showing: 61 - 80 of 894 results